The Alpaca Melanocortin 1 Receptor: Gene Mutations, Transcripts, and Relative Levels of Expression in Ventral Skin Biopsies
Joint Authors
Chandramohan, Bathrachalam
Renieri, Carlo
La Manna, Vincenzo
La Terza, Antonietta
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-05
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
The objectives of the present study were to characterize the MC1R gene, its transcripts and the single nucleotide polymorphisms (SNPs) associated with coat color in alpaca.
Full length cDNA amplification revealed the presence of two transcripts, named as F1 and F2, differing only in the length of their 5′-terminal untranslated region (UTR) sequences and presenting a color specific expression.
Whereas the F1 transcript was common to white and colored (black and brown) alpaca phenotypes, the shorter F2 transcript was specific to white alpaca.
Further sequencing of the MC1R gene in white and colored alpaca identified a total of twelve SNPs; among those nine (four silent mutations (c.126C>A, c.354T>C, c.618G>A, and c.933G>A); five missense mutations (c.82A>G, c.92C>T, c.259A>G, c.376A>G, and c.901C>T)) were observed in coding region and three in the 3′UTR.
A 4 bp deletion (c.224 227del) was also identified in the coding region.
Molecular segregation analysis uncovered that the combinatory mutations in the MC1R locus could cause eumelanin and pheomelanin synthesis in alpaca.
Overall, our data refine what is known about the MC1R gene and provides additional information on its role in alpaca pigmentation.
American Psychological Association (APA)
Chandramohan, Bathrachalam& Renieri, Carlo& La Manna, Vincenzo& La Terza, Antonietta. 2015. The Alpaca Melanocortin 1 Receptor: Gene Mutations, Transcripts, and Relative Levels of Expression in Ventral Skin Biopsies. The Scientific World Journal،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1078626
Modern Language Association (MLA)
Chandramohan, Bathrachalam…[et al.]. The Alpaca Melanocortin 1 Receptor: Gene Mutations, Transcripts, and Relative Levels of Expression in Ventral Skin Biopsies. The Scientific World Journal No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1078626
American Medical Association (AMA)
Chandramohan, Bathrachalam& Renieri, Carlo& La Manna, Vincenzo& La Terza, Antonietta. The Alpaca Melanocortin 1 Receptor: Gene Mutations, Transcripts, and Relative Levels of Expression in Ventral Skin Biopsies. The Scientific World Journal. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1078626
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1078626