Genetic engineering to improve β-carotene content in pepper

Joint Authors

al-Najjar, Mahran Mukhtar
Makkawi, Inas Mahmud

Source

Annals of Agricultural Science, Moshtohor

Publisher

Banha University Faculty of Agriculture

Publication Date

2018-04-30

Country of Publication

Egypt

No. of Pages

7

Main Subjects

Agriculture

Topics

• Genetic engineering
• Wild plants
• Analytical chemistry
• Transgenic plants

English Abstract

A laboratory study was conducted to develop a broadly applicable in vitro regeneration and transformation methods for increasing β-carotene content in pepper fruits.

Therefore, two different pepper genotypes were analyzed with regard to their efficiency for regeneration and genetic transformation in vitro.

Cotyledons were used in vitro from young seedlings as target tissue for regeneration and transformation of pepper.

Over expression of β-Lcy gene via Agrobacterium-mediated transformation was used.

The presence of the transgene was assayed on leaf DNA via PCR and biochemical analysis using HPLC.

Results strongly showed that there were significant differences between pepper genotypes.

The transgenic pepper plants showed a significant increase in fruit β-carotene content reaching 7 to 10 folds according to the genotype.

The fruits from these plants displayed different colour phenotypes, from orange to orange-red.

The difference in the fruit colour phenotypes reflects the beta-carotene ratio, which ranges from 4.5 mg/100g dw in the wild plants to 45.0 in the transgenic plants of genotype Balady during maturity stage.

Concerning to the genotype Topepo rosso, the β-carotene content was increased from 5.2 mg/100g dw in the wild plants to 36.39 mg/100g dw in the transgenic plants during mature stage.

Data Type

Conference Papers

Record ID

BIM-1093702

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