Metformin Alleviated Aβ-Induced Apoptosis via the Suppression of JNK MAPK Signaling Pathway in Cultured Hippocampal Neurons
Joint Authors
Chen, Bin
Teng, Ying
Zhang, Xingguang
Lv, Xiaofeng
Yin, Yanling
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Both diabetes and hyperinsulinemia are confirmed risk factors for Alzheimer’s disease.
Some researchers proposed that antidiabetic drugs may be used as disease-modifying therapies, such as metformin and thiazolidinediones, although more evidence was poorly supported.
The aim of the current study is to investigate the role of metformin in Aβ-induced cytotoxicity and explore the underlying mechanisms.
First, the experimental results show that metformin salvaged the neurons exposed to Aβ in a concentration-dependent manner with MTT and LDH assay.
Further, the phosphorylation levels of JNK, ERK1/2, and p38 MAPK were measured with western blot analysis.
It was investigated that Aβ increased phospho-JNK significantly but had no effect on phospho-p38 MAPK and phospho-ERK1/2.
Metformin decreased hyperphosphorylated JNK induced by Aβ; however, the protection of metformin against Aβ was blocked when anisomycin, the activator of JNK, was added to the medium, indicating that metformin performed its protection against Aβ in a JNK-dependent way.
In addition, it was observed that metformin protected the neurons via the suppression of apoptosis.
Taken together, our findings demonstrate that metformin may have a positive effect on Aβ-induced cytotoxicity, which provides a preclinical strategy against AD for elders with diabetes.
American Psychological Association (APA)
Chen, Bin& Teng, Ying& Zhang, Xingguang& Lv, Xiaofeng& Yin, Yanling. 2016. Metformin Alleviated Aβ-Induced Apoptosis via the Suppression of JNK MAPK Signaling Pathway in Cultured Hippocampal Neurons. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1096874
Modern Language Association (MLA)
Chen, Bin…[et al.]. Metformin Alleviated Aβ-Induced Apoptosis via the Suppression of JNK MAPK Signaling Pathway in Cultured Hippocampal Neurons. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1096874
American Medical Association (AMA)
Chen, Bin& Teng, Ying& Zhang, Xingguang& Lv, Xiaofeng& Yin, Yanling. Metformin Alleviated Aβ-Induced Apoptosis via the Suppression of JNK MAPK Signaling Pathway in Cultured Hippocampal Neurons. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1096874
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1096874