Effects of Berberine on NLRP3 and IL-1β Expressions in Monocytic THP-1 Cells with Monosodium Urate Crystals-Induced Inflammation

Abstract EN

Background.

Urate crystals-induced inflammation is a critical factor during the initiation of gouty arthritis.

Berberine is well known for its anti-inflammatory activity.

However, the underlying effects of berberine on monosodium urate crystals-induced inflammation remain obscure.

Objectives.

This study is set to explore the protective effect and mechanism of berberine on monosodium urate crystals-induced inflammation in human monocytic THP-1 cells.

Methods.

The mRNA levels of NLRP3 and IL-1β were measured by Real-Time PCR, and the protein levels of NLRP3 and IL-1β were determined by ELISA, Western blot, and immunofluorescence.

Results.

The NLRP3 and IL-1β expressions were significantly increased in model group compared to that in normal group ( P < 0.05 ).

Meanwhile, there was significant reduction in the expressions of NLRP3 and IL-1β mRNA in groups 6.25 μM berberine and 25 μM berberine when compared with model group ( P < 0.05 ).

Conclusions.

Therefore, berberine alleviates monosodium urate crystals-induced inflammation by downregulating NLRP3 and IL-1β expressions.

The regulatory effects of berberine may be related to the inactivation of NLRP3 inflammasome.