Effects of Berberine on NLRP3 and IL-1β Expressions in Monocytic THP-1 Cells with Monosodium Urate Crystals-Induced Inflammation
Joint Authors
Liu, Yafei
Chen, Zhe
Wen, Cai-Yu-Zhu
Huang, Ying
Tu, Shenghao
Wang, Yu
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-05
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
Urate crystals-induced inflammation is a critical factor during the initiation of gouty arthritis.
Berberine is well known for its anti-inflammatory activity.
However, the underlying effects of berberine on monosodium urate crystals-induced inflammation remain obscure.
Objectives.
This study is set to explore the protective effect and mechanism of berberine on monosodium urate crystals-induced inflammation in human monocytic THP-1 cells.
Methods.
The mRNA levels of NLRP3 and IL-1β were measured by Real-Time PCR, and the protein levels of NLRP3 and IL-1β were determined by ELISA, Western blot, and immunofluorescence.
Results.
The NLRP3 and IL-1β expressions were significantly increased in model group compared to that in normal group ( P < 0.05 ).
Meanwhile, there was significant reduction in the expressions of NLRP3 and IL-1β mRNA in groups 6.25 μM berberine and 25 μM berberine when compared with model group ( P < 0.05 ).
Conclusions.
Therefore, berberine alleviates monosodium urate crystals-induced inflammation by downregulating NLRP3 and IL-1β expressions.
The regulatory effects of berberine may be related to the inactivation of NLRP3 inflammasome.
American Psychological Association (APA)
Liu, Yafei& Wen, Cai-Yu-Zhu& Chen, Zhe& Wang, Yu& Huang, Ying& Tu, Shenghao. 2016. Effects of Berberine on NLRP3 and IL-1β Expressions in Monocytic THP-1 Cells with Monosodium Urate Crystals-Induced Inflammation. BioMed Research International،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1097088
Modern Language Association (MLA)
Liu, Yafei…[et al.]. Effects of Berberine on NLRP3 and IL-1β Expressions in Monocytic THP-1 Cells with Monosodium Urate Crystals-Induced Inflammation. BioMed Research International No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1097088
American Medical Association (AMA)
Liu, Yafei& Wen, Cai-Yu-Zhu& Chen, Zhe& Wang, Yu& Huang, Ying& Tu, Shenghao. Effects of Berberine on NLRP3 and IL-1β Expressions in Monocytic THP-1 Cells with Monosodium Urate Crystals-Induced Inflammation. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1097088
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097088