Overexpression of Prolyl-4-Hydroxylase-α1 Stabilizes but Increases Shear Stress-Induced Atherosclerotic Plaque in Apolipoprotein E-Deficient Mice
Joint Authors
Cao, Xiao-qing
Liu, Xin-xin
Li, Meng-meng
Zhang, Yu
Chen, Liang
Wang, Lin
Di, Ming-xue
Zhang, Mei
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-12
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The rupture and erosion of atherosclerotic plaque can induce coronary thrombosis.
Prolyl-4-hydroxylase (P4H) plays a central role in the synthesis of all known types of collagens, which are the most abundant constituent of the extracellular matrix in atherosclerotic plaque.
The pathogenesis of atherosclerosis is thought to be in part caused by shear stress.
In this study, we aimed to investigate a relationship between P4Hα1 and shear stress-induced atherosclerotic plaque.
Carotid arteries of ApoE−/− mice were exposed to low and oscillatory shear stress conditions by the placement of a shear stress cast for 2 weeks; we divided 60 male ApoE−/− mice into three groups for treatments with saline (mock) (n=20), empty lentivirus (lenti-EGFP) (n=20), and lentivirus-P4Hα1 (lenti-P4Hα1) (n=20).
Our results reveal that after 2 weeks of lenti-P4Hα1 treatment both low and oscillatory shear stress-induced plaques increased collagen and the thickness of fibrous cap and decreased macrophage accumulation but no change in lipid accumulation.
We also observed that overexpression of P4Ha1 increased plaque size.
Our study suggests that P4Hα1 overexpression might be a potential therapeutic target in stabilizing vulnerable plaques.
American Psychological Association (APA)
Cao, Xiao-qing& Liu, Xin-xin& Li, Meng-meng& Zhang, Yu& Chen, Liang& Wang, Lin…[et al.]. 2016. Overexpression of Prolyl-4-Hydroxylase-α1 Stabilizes but Increases Shear Stress-Induced Atherosclerotic Plaque in Apolipoprotein E-Deficient Mice. Disease Markers،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1103660
Modern Language Association (MLA)
Cao, Xiao-qing…[et al.]. Overexpression of Prolyl-4-Hydroxylase-α1 Stabilizes but Increases Shear Stress-Induced Atherosclerotic Plaque in Apolipoprotein E-Deficient Mice. Disease Markers No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1103660
American Medical Association (AMA)
Cao, Xiao-qing& Liu, Xin-xin& Li, Meng-meng& Zhang, Yu& Chen, Liang& Wang, Lin…[et al.]. Overexpression of Prolyl-4-Hydroxylase-α1 Stabilizes but Increases Shear Stress-Induced Atherosclerotic Plaque in Apolipoprotein E-Deficient Mice. Disease Markers. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1103660
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1103660