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Reassessing the Role of the Active TGF-β1 as a Biomarker in Systemic Sclerosis: Association of Serum Levels with Clinical Manifestations
Joint Authors
Pitta, Ivan da Rocha
Pereira, Michelly Cristiny
Gonçalves, Sayonara Maria Calado
Marques, Claudia Diniz Lopes
Almeida, Anderson Rodrigues de
Gonçalves, Rafaela Silva Guimarães
Sampaio, Maria Clara Pinheiro Duarte
Vilar, Kamila de Melo
Rêgo, Moacyr Jesus Barreto de Melo
Pitta, Maira Galdino da Rocha
Dantas, Andréa Tavares
Duarte, Ângela Luzia Branco Pinto
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-14
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Objective.
To determine active TGF-β1 (aTGF-β1) levels in serum, skin, and peripheral blood mononuclear cell (PBMC) culture supernatants and to understand their associations with clinical parameters in systemic sclerosis (SSc) patients.
Methods.
We evaluated serum samples from 56 SSc patients and 24 healthy controls (HC).
In 20 SSc patients, we quantified spontaneous or anti-CD3/CD28 stimulated production of aTGF-β1 by PBMC.
The aTGF-β1 levels were measured by ELISA.
Skin biopsies were obtained from 13 SSc patients and six HC, and TGFB1 expression was analyzed by RT-PCR.
Results.
TGF-β1 serum levels were significantly higher in SSc patients than in HC (p < 0.0001).
Patients with increased TGF-β1 serum levels were more likely to have diffuse subset (p = 0.02), digital ulcers (p = 0.02), lung fibrosis (p < 0.0001), positive antitopoisomerase I (p = 0.03), and higher modified Rodnan score (p = 0.046).
Most of our culture supernatant samples had undetectable levels of TGF-β1.
No significant difference in TGFB1 expression was observed in the SSc skin compared with HC skin.
Conclusion.
Raised active TGF-β1 serum levels and their association with clinical manifestations in scleroderma patients suggest that this cytokine could be a marker of fibrotic and vascular involvement in SSc.
American Psychological Association (APA)
Dantas, Andréa Tavares& Gonçalves, Sayonara Maria Calado& Almeida, Anderson Rodrigues de& Gonçalves, Rafaela Silva Guimarães& Sampaio, Maria Clara Pinheiro Duarte& Vilar, Kamila de Melo…[et al.]. 2016. Reassessing the Role of the Active TGF-β1 as a Biomarker in Systemic Sclerosis: Association of Serum Levels with Clinical Manifestations. Disease Markers،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1103737
Modern Language Association (MLA)
Dantas, Andréa Tavares…[et al.]. Reassessing the Role of the Active TGF-β1 as a Biomarker in Systemic Sclerosis: Association of Serum Levels with Clinical Manifestations. Disease Markers No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1103737
American Medical Association (AMA)
Dantas, Andréa Tavares& Gonçalves, Sayonara Maria Calado& Almeida, Anderson Rodrigues de& Gonçalves, Rafaela Silva Guimarães& Sampaio, Maria Clara Pinheiro Duarte& Vilar, Kamila de Melo…[et al.]. Reassessing the Role of the Active TGF-β1 as a Biomarker in Systemic Sclerosis: Association of Serum Levels with Clinical Manifestations. Disease Markers. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1103737
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1103737