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Influence of βS-Globin Haplotypes and Hydroxyurea on Arginase I Levels in Sickle Cell Disease
Joint Authors
Moreira, J. A.
Machado, R. P. G.
Laurentino, M. R.
Lemes, Romelia Pinheiro Gonçalves
Barbosa, M. C.
Santos, T. E.
Bandeira, I. C. J.
Martins, Alice Maria Costa
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-04
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Introduction.
Sickle cell disease (SCD) is characterized by hemoglobin S homozygosity, leading to hemolysis and vasoocclusion.
The hemolysis releases arginase I, an enzyme that decreases the bioavailability of nitric oxide, worsening the symptoms.
The different SCD haplotypes are related to clinical symptoms and varied hemoglobin F (HbF) concentration.
The aim of this study was to evaluate the impact of the βS gene haplotypes and HbF concentration on arginase I levels in SCD patients.
Methods.
Fifty SCD adult patients were enrolled in the study and 20 blood donors composed the control group.
Arginase I was measured by ELISA.
The βS haplotypes were identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
Statistical analyses were performed with GraphPad Prism program and the significance level was p < 0.05 .
Results.
Significant increase was observed in the arginase I levels in SCD patients compared to the control group ( p < 0.0001 ).
The comparison between the levels of arginase I in three haplotypes groups showed a difference between the Bantu/Bantu × Bantu/Benin groups; Bantu/Bantu × Benin/Benin, independent of HU dosage.
An inverse correlation with the arginase I levels and HbF concentration was observed.
Conclusion.
The results support the hypothesis that arginase I is associated with HbF concentration, also measured indirectly by the association with haplotypes.
American Psychological Association (APA)
Moreira, J. A.& Machado, R. P. G.& Laurentino, M. R.& Lemes, Romelia Pinheiro Gonçalves& Barbosa, M. C.& Santos, T. E.…[et al.]. 2016. Influence of βS-Globin Haplotypes and Hydroxyurea on Arginase I Levels in Sickle Cell Disease. Disease Markers،Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1103806
Modern Language Association (MLA)
Moreira, J. A.…[et al.]. Influence of βS-Globin Haplotypes and Hydroxyurea on Arginase I Levels in Sickle Cell Disease. Disease Markers No. 2016 (2016), pp.1-5.
https://search.emarefa.net/detail/BIM-1103806
American Medical Association (AMA)
Moreira, J. A.& Machado, R. P. G.& Laurentino, M. R.& Lemes, Romelia Pinheiro Gonçalves& Barbosa, M. C.& Santos, T. E.…[et al.]. Influence of βS-Globin Haplotypes and Hydroxyurea on Arginase I Levels in Sickle Cell Disease. Disease Markers. 2016. Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1103806
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1103806