Identification of Centella asiatica’s Effective Ingredients for Inducing the Neuronal Differentiation
Joint Authors
Jiang, Hui
Zheng, Guoshuai
Lv, Junwei
Chen, Heyu
Lin, Jinjin
Li, Yiyang
Fan, Guorong
Ding, Xianting
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-30
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Centella asiatica, commonly known as Gotu kola, has been widely used as a traditional herb for decades.
Yet, the study on which compounds or compound combinations actually lead to its brain benefits remains scarce.
To study the neuroprotection effects of Centella asiatica, neuronal differentiation of PC12 cells was applied.
In our pilot study, we isolated 45 Centella asiatica fractions and tested their abilities for inducing neuronal differentiation on PC12 cells.
The most effective fraction showed robust induction in neurite outgrowth and neurofilament expression.
LC-MS fingerprint analysis of this fraction revealed asiatic acid and madecassic acid as the dominant components.
A further investigation on the pure combination of these two compounds indicated that the combination of these two compounds extensively promoted nerve differentiation in vitro.
Application of PD98059, a protein MEK inhibitor, attenuated combination-induced neurofilament expression, indicating the combination-induced nerve differentiation through activation of MEK signaling pathway.
Our results support the use of combination of asiatic acid and madecassic acid as an effective mean to intervene neurodegenerative diseases in which neurotrophin deficiency is involved.
American Psychological Association (APA)
Jiang, Hui& Zheng, Guoshuai& Lv, Junwei& Chen, Heyu& Lin, Jinjin& Li, Yiyang…[et al.]. 2016. Identification of Centella asiatica’s Effective Ingredients for Inducing the Neuronal Differentiation. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1104523
Modern Language Association (MLA)
Jiang, Hui…[et al.]. Identification of Centella asiatica’s Effective Ingredients for Inducing the Neuronal Differentiation. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1104523
American Medical Association (AMA)
Jiang, Hui& Zheng, Guoshuai& Lv, Junwei& Chen, Heyu& Lin, Jinjin& Li, Yiyang…[et al.]. Identification of Centella asiatica’s Effective Ingredients for Inducing the Neuronal Differentiation. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1104523
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104523