Simultaneous Determination of Four Active Ingredients in Sargentodoxa cuneata by HPLC Coupled with Evaporative Light Scattering Detection
Joint Authors
Li, Di-Hua
Wang, Yan
Lv, Yuan-Shan
Liu, Jun-Hong
Yang, Lei
Zhuo, Yu-Zhen
Zhang, Shu-Kun
Source
International Journal of Analytical Chemistry
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-29
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
A HPLC coupled with evaporative light scattering detection method had been developed for the simultaneous determination of 3,4-dihydroxyphenylethyl alcohol glycoside, salidroside, chlorogenic acid, and liriodendrin in the stem of Sargentodoxa cuneata.
With a C18 column, the analysis was performed using acetonitrile and 0.2% formic acid aqueous solution as mobile phase in gradient program at a flow rate of 0.9 mL/min.
The optimum drift tube temperature of evaporative light scattering detection was at 105°C with the air flow rate of 2.5 L/min.
The calibration curves showed good linearity during the test ranges.
This method was validated for limits of detection and quantification, precision, and reproducibility.
The recoveries were within the range of 96.39%–104.64%.
The relative standard deviations of intraday and interday precision were less than 2.90% and 3.30%, respectively.
The developed method can be successfully used to quantify the four analytes in the stem of Sargentodoxa cuneata from various regions in China.
American Psychological Association (APA)
Li, Di-Hua& Lv, Yuan-Shan& Liu, Jun-Hong& Yang, Lei& Wang, Yan& Zhang, Shu-Kun…[et al.]. 2016. Simultaneous Determination of Four Active Ingredients in Sargentodoxa cuneata by HPLC Coupled with Evaporative Light Scattering Detection. International Journal of Analytical Chemistry،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1104958
Modern Language Association (MLA)
Li, Di-Hua…[et al.]. Simultaneous Determination of Four Active Ingredients in Sargentodoxa cuneata by HPLC Coupled with Evaporative Light Scattering Detection. International Journal of Analytical Chemistry No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1104958
American Medical Association (AMA)
Li, Di-Hua& Lv, Yuan-Shan& Liu, Jun-Hong& Yang, Lei& Wang, Yan& Zhang, Shu-Kun…[et al.]. Simultaneous Determination of Four Active Ingredients in Sargentodoxa cuneata by HPLC Coupled with Evaporative Light Scattering Detection. International Journal of Analytical Chemistry. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1104958
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104958