A High-Throughput Size Exclusion Chromatography Method to Determine the Molecular Size Distribution of Meningococcal Polysaccharide Vaccine
Joint Authors
Khan, Imran
Rahman, K. M. Taufiqur
Siraj, S. M. Saad Us
Karim, Mahbubul
Muktadir, Abdul
Maheshwari, Arpan
Kabir, Md Azizul
Nahar, Zebun
Ahasan, Mohammad Mainul
Source
International Journal of Analytical Chemistry
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-05
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Molecular size distribution of meningococcal polysaccharide vaccine is a readily identifiable parameter that directly correlates with the immunogenicity.
In this paper, we report a size exclusion chromatography method to determine the molecular size distribution and distribution coefficient value of meningococcal polysaccharide serogroups A, C, W, and Y in meningococcal polysaccharide (ACWY) vaccines.
The analyses were performed on a XK16/70 column packed with sepharose CL-4B with six different batches of Ingovax® ACWY, a meningococcal polysaccharide vaccine produced by Incepta Vaccine Ltd., Bangladesh.
A quantitative rocket immunoelectrophoresis assay was employed to determine the polysaccharide contents of each serogroup.
The calculated distribution coefficient values of serogroups A, C, W, and Y were found to be 0.26 ± 0.16 , 0.21 ± 0.11 , 0.21 ± 0.11 , and 0.14 ± 0.12 , respectively, and met the requirements of British Pharmacopeia.
The method was proved to be robust for determining the distribution coefficient values which is an obligatory requirement for vaccine lot release.
American Psychological Association (APA)
Khan, Imran& Rahman, K. M. Taufiqur& Siraj, S. M. Saad Us& Karim, Mahbubul& Muktadir, Abdul& Maheshwari, Arpan…[et al.]. 2016. A High-Throughput Size Exclusion Chromatography Method to Determine the Molecular Size Distribution of Meningococcal Polysaccharide Vaccine. International Journal of Analytical Chemistry،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1104960
Modern Language Association (MLA)
Khan, Imran…[et al.]. A High-Throughput Size Exclusion Chromatography Method to Determine the Molecular Size Distribution of Meningococcal Polysaccharide Vaccine. International Journal of Analytical Chemistry No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1104960
American Medical Association (AMA)
Khan, Imran& Rahman, K. M. Taufiqur& Siraj, S. M. Saad Us& Karim, Mahbubul& Muktadir, Abdul& Maheshwari, Arpan…[et al.]. A High-Throughput Size Exclusion Chromatography Method to Determine the Molecular Size Distribution of Meningococcal Polysaccharide Vaccine. International Journal of Analytical Chemistry. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1104960
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104960