Microwave-Assisted Tissue Preparation for Rapid Fixation, Decalcification, Antigen Retrieval, Cryosectioning, and Immunostaining
Author
Source
International Journal of Cell Biology
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-20
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Microwave irradiation of tissue during fixation and subsequent histochemical staining procedures significantly reduces the time required for incubation in fixation and staining solutions.
Minimizing the incubation time in fixative reduces disruption of tissue morphology, and reducing the incubation time in staining solution or antibody solution decreases nonspecific labeling.
Reduction of incubation time in staining solution also decreases the level of background noise.
Microwave-assisted tissue preparation is applicable for tissue fixation, decalcification of bone tissues, treatment of adipose tissues, antigen retrieval, and other special staining of tissues.
Microwave-assisted tissue fixation and staining are useful tools for histological analyses.
This review describes the protocols using microwave irradiation for several essential procedures in histochemical studies, and these techniques are applicable to other protocols for tissue fixation and immunostaining in the field of cell biology.
American Psychological Association (APA)
Katoh, Kazuo. 2016. Microwave-Assisted Tissue Preparation for Rapid Fixation, Decalcification, Antigen Retrieval, Cryosectioning, and Immunostaining. International Journal of Cell Biology،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1105506
Modern Language Association (MLA)
Katoh, Kazuo. Microwave-Assisted Tissue Preparation for Rapid Fixation, Decalcification, Antigen Retrieval, Cryosectioning, and Immunostaining. International Journal of Cell Biology No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1105506
American Medical Association (AMA)
Katoh, Kazuo. Microwave-Assisted Tissue Preparation for Rapid Fixation, Decalcification, Antigen Retrieval, Cryosectioning, and Immunostaining. International Journal of Cell Biology. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1105506
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1105506