ClC-3 Expression and Its Association with Hyperglycemia Induced HT22 Hippocampal Neuronal Cell Apoptosis
Joint Authors
Wang, Xin
Fan, Feiyan
Liu, Tao
Ren, Dongni
Liu, Hui
Zhang, Pengxing
Wang, Zhen
Liu, Nan
Li, Qian
Fu, Jianfang
Tu, Yanyang
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-26
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Although apoptosis plays an important role in the development of Diabetic Encephalopathy (DE), the underlying molecular mechanisms remain unclear.
With respect to this, the present work aims to study the variation in chloride/proton exchanger ClC-3 expression and its association with HT22 hippocampal neuronal apoptosis under hyperglycemic condition in vitro.
The cells were stimulated with added 0, 5, or 25 mM glucose or mannitol for up to 72 hours before assessing the rate of ClC-3 expression, cell viability, and apoptosis.
In a consecutive experiment, cells received chloride channel blocker in addition to glucose.
The rate of cellular death/apoptosis and viability was measured using Flow Cytometry and MTT assay, respectively.
Changes in ClC-3 expression were assessed using immunofluorescence staining and western blot analysis.
The results revealed a significant increase in cellular apoptosis and reduction in viability, associated with increased ClC-3 expression in high glucose group.
Osmolarity had no role to play.
Addition of chloride channel blocker completely abolished this effect.
Thus we conclude that, with its increased expression, ClC-3 plays a major role in hyperglycemia induced hippocampal neuronal apoptosis.
To strengthen our understanding of this aforesaid association, we conducted an extensive literature search which is presented in this paper.
American Psychological Association (APA)
Fan, Feiyan& Liu, Tao& Wang, Xin& Ren, Dongni& Liu, Hui& Zhang, Pengxing…[et al.]. 2016. ClC-3 Expression and Its Association with Hyperglycemia Induced HT22 Hippocampal Neuronal Cell Apoptosis. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1108046
Modern Language Association (MLA)
Fan, Feiyan…[et al.]. ClC-3 Expression and Its Association with Hyperglycemia Induced HT22 Hippocampal Neuronal Cell Apoptosis. Journal of Diabetes Research No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1108046
American Medical Association (AMA)
Fan, Feiyan& Liu, Tao& Wang, Xin& Ren, Dongni& Liu, Hui& Zhang, Pengxing…[et al.]. ClC-3 Expression and Its Association with Hyperglycemia Induced HT22 Hippocampal Neuronal Cell Apoptosis. Journal of Diabetes Research. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1108046
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108046