Exendin-4 Promotes Survival of Mouse Pancreatic β-Cell Line in Lipotoxic Conditions, through the Extracellular Signal-Related Kinase 12 Pathway
Joint Authors
Gu, Jianqiu
Zheng, Hongzhi
Wei, Qian
Meng, Xin
Zhang, Jin
Wang, Difei
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-30
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Type 2 diabetes is a heterogeneous disorder that develops as a result of relatively inappropriate insulin secretion and insulin resistance.
Increased levels of free fatty acids (FFAs) are one of the important factors for the pathogenesis of type 2 diabetes and contribute to defective β-cell proliferation and increased β-cell apoptosis.
Recently, glucagon-like peptide-1 (GLP-1) receptor agonists have been shown to possess an antiapoptotic effect, by increasing β-cell mass and improving β-cell function.
However, their effects on β-cells in vitro against lipotoxicity have not been elucidated completely.
In this study, we investigated whether the GLP-1 receptor agonist exendin-4 displays prosurvival effects in pancreatic β-cells exposed to chronic elevated FFAs.
Results showed that exendin-4 inhibited apoptosis induced by palmitate in MIN6 cells.
After 24 h of incubation, exendin-4 caused rapid activation of extracellular signal-related kinase 1/2 (ERK1/2) under lipotoxic conditions.
The ERK1/2 inhibitor PD98059 blocked the antilipotoxic effect of exendin-4 on MIN6 cells.
Exendin-4 also inhibited the mitochondrial pathway of apoptosis.
This inhibition is associated with upregulation of BCL-2.
Our findings suggested that exendin-4 may exert cytoprotective effects through activation of ERK1/2 and inhibition of the mitochondrial apoptosis pathway.
American Psychological Association (APA)
Gu, Jianqiu& Wei, Qian& Zheng, Hongzhi& Meng, Xin& Zhang, Jin& Wang, Difei. 2016. Exendin-4 Promotes Survival of Mouse Pancreatic β-Cell Line in Lipotoxic Conditions, through the Extracellular Signal-Related Kinase 12 Pathway. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1108148
Modern Language Association (MLA)
Gu, Jianqiu…[et al.]. Exendin-4 Promotes Survival of Mouse Pancreatic β-Cell Line in Lipotoxic Conditions, through the Extracellular Signal-Related Kinase 12 Pathway. Journal of Diabetes Research No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1108148
American Medical Association (AMA)
Gu, Jianqiu& Wei, Qian& Zheng, Hongzhi& Meng, Xin& Zhang, Jin& Wang, Difei. Exendin-4 Promotes Survival of Mouse Pancreatic β-Cell Line in Lipotoxic Conditions, through the Extracellular Signal-Related Kinase 12 Pathway. Journal of Diabetes Research. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1108148
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108148