A Novel Wistar Rat Model of Obesity-Related Nonalcoholic Fatty Liver Disease Induced by Sucrose-Rich Diet
Joint Authors
Lima, Maria Luíza R. P.
Leite, Laura H. R.
Gioda, Carolina R.
Leme, Fabíola O. P.
Couto, Claudia A.
Coimbra, Cândido C.
Leite, Virginia H. R.
Ferrari, Teresa Cristina A.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The pathogenesis of nonalcoholic fatty liver disease (NAFLD) is not fully understood, and experimental models are an alternative to study this issue.
We investigated the effects of a simple carbohydrate-rich diet on the development of obesity-related NAFLD and the impact of physical training on the metabolic abnormalities associated with this disorder.
Sixty Wistar rats were randomly separated into experimental and control groups, which were fed with sucrose-enriched (18% simple carbohydrates) and standard diet, respectively.
At the end of each experimental period (5, 10, 20, and 30 weeks), 6 animals from each group were sacrificed for blood tests and liver histology and immunohistochemistry.
From weeks 25 to 30, 6 animals from each group underwent physical training.
The experimental group animals developed obesity and NAFLD, characterized histopathologically by steatosis and hepatocellular ballooning, clinically by increased thoracic circumference and body mass index associated with hyperleptinemia, and metabolically by hyperglycemia, hyperinsulinemia, hypertriglyceridemia, increased levels of very low-density lipoprotein- (VLDL-) cholesterol, depletion of the antioxidants liver enzymes superoxide dismutase and catalase, and increased hepatic levels of malondialdehyde, an oxidative stress marker.
Rats that underwent physical training showed increased high-density lipoprotein- (HDL-) cholesterol levels.
In conclusion, a sucrose-rich diet induced obesity, insulin resistance, oxidative stress, and NAFLD in rats.
American Psychological Association (APA)
Lima, Maria Luíza R. P.& Leite, Laura H. R.& Gioda, Carolina R.& Leme, Fabíola O. P.& Couto, Claudia A.& Coimbra, Cândido C.…[et al.]. 2015. A Novel Wistar Rat Model of Obesity-Related Nonalcoholic Fatty Liver Disease Induced by Sucrose-Rich Diet. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1108314
Modern Language Association (MLA)
Lima, Maria Luíza R. P.…[et al.]. A Novel Wistar Rat Model of Obesity-Related Nonalcoholic Fatty Liver Disease Induced by Sucrose-Rich Diet. Journal of Diabetes Research No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1108314
American Medical Association (AMA)
Lima, Maria Luíza R. P.& Leite, Laura H. R.& Gioda, Carolina R.& Leme, Fabíola O. P.& Couto, Claudia A.& Coimbra, Cândido C.…[et al.]. A Novel Wistar Rat Model of Obesity-Related Nonalcoholic Fatty Liver Disease Induced by Sucrose-Rich Diet. Journal of Diabetes Research. 2015. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1108314
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108314