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Nerve Growth Factor Regulation by TNF-α and IL-1β in Synovial Macrophages and Fibroblasts in Osteoarthritic Mice
Joint Authors
Uchida, Kentaro
Aikawa, Jun
Takano, Shotaro
Inoue, Gen
Fujimaki, Hisako
Iwase, Dai
Minatani, Atsushi
Miyagi, Masayuki
Takaso, Masashi
Iwabuchi, Kazuya
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-18
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
To investigate the role of macrophages as a regulator and producer of nerve growth factor (NGF) in the synovial tissue (ST) of osteoarthritis (OA) joints, the gene expression profiles of several inflammatory cytokines in the ST, including synovial macrophages and fibroblasts, of OA mice (STR/Ort) were characterized.
Specifically, real-time polymerase chain reaction analysis was used to evaluate the expression of tumor necrosis factor- (TNF-) α, interleukin- (IL-) 1β, IL-6, and NGF in CD11b+ and CD11b– cells isolated from the ST of a murine OA model.
The effects of TNF-α, IL-1β, and IL-6 on the expression of NGF in cultured synovial cells were also examined.
The expression of TNF-α, IL-1β, IL-6, and NGF in the ST of STR/Ort was higher than that in C57/BL6J mice.
Compared to the CD11b– cell fraction, higher expression levels of TNF-α, IL-1β, and IL-6 were detected in the CD11b+ cell fraction, whereas no differences in the expression of NGF were detected between the two cell fractions.
Notably, TNF-α upregulated NGF expression in synovial fibroblasts and macrophages and IL-1β upregulated NGF expression in synovial fibroblasts.
IL-1β and TNF-α may regulate NGF signaling in OA joints and be suitable therapeutic targets for treating OA pain.
American Psychological Association (APA)
Takano, Shotaro& Uchida, Kentaro& Miyagi, Masayuki& Inoue, Gen& Fujimaki, Hisako& Aikawa, Jun…[et al.]. 2016. Nerve Growth Factor Regulation by TNF-α and IL-1β in Synovial Macrophages and Fibroblasts in Osteoarthritic Mice. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1108827
Modern Language Association (MLA)
Takano, Shotaro…[et al.]. Nerve Growth Factor Regulation by TNF-α and IL-1β in Synovial Macrophages and Fibroblasts in Osteoarthritic Mice. Journal of Immunology Research No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1108827
American Medical Association (AMA)
Takano, Shotaro& Uchida, Kentaro& Miyagi, Masayuki& Inoue, Gen& Fujimaki, Hisako& Aikawa, Jun…[et al.]. Nerve Growth Factor Regulation by TNF-α and IL-1β in Synovial Macrophages and Fibroblasts in Osteoarthritic Mice. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1108827
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108827