Development of Immunocapture-LCMS Assay for Simultaneous ADA Isotyping and Semiquantitation
Joint Authors
Chen, Lin-Zhi
Roos, David
Philip, Elsy
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-28
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Therapeutic proteins and peptides have potential to elicit immune responses resulting in anti-drug antibodies that can pose problems for both patient safety and product efficacy.
During drug development immunogenicity is usually examined by risk-based approach along with specific strategies for developing “fit-for-purpose” bioanalytical approaches.
Enzyme-linked immunosorbent assays and electrochemiluminescence immunoassays are the most widely used platform for ADA detection due to their high sensitivity and throughput.
During the past decade, LC/MS has emerged as a promising technology for quantitation of biotherapeutics and protein biomarkers in biological matrices, mainly owing to its high specificity, selectivity, multiplexing, and wide dynamic range.
In fully taking these advantages, we describe here an immunocapture-LC/MS methodology for simultaneous isotyping and semiquantitation of ADA in human plasma.
Briefly, ADA and/or drug-ADA complex is captured by biotinylated drug or anti-drug Ab, immobilized on streptavidin magnetic beads, and separated from human plasma by a magnet.
ADA is then released from the beads and subjected to trypsin digestion followed by LC/MS detection of specific universal peptides for each ADA isotype.
The LC/MS data are analyzed using cut-point and calibration curve.
The proof-of-concept of this methodology is demonstrated by detecting preexisting ADA in human plasma.
American Psychological Association (APA)
Chen, Lin-Zhi & Roos, David& Philip, Elsy. 2016. Development of Immunocapture-LCMS Assay for Simultaneous ADA Isotyping and Semiquantitation. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1108879
Modern Language Association (MLA)
Chen, Lin-Zhi…[et al.]. Development of Immunocapture-LCMS Assay for Simultaneous ADA Isotyping and Semiquantitation. Journal of Immunology Research No. 2016 (2016), pp.1-14.
https://search.emarefa.net/detail/BIM-1108879
American Medical Association (AMA)
Chen, Lin-Zhi & Roos, David& Philip, Elsy. Development of Immunocapture-LCMS Assay for Simultaneous ADA Isotyping and Semiquantitation. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1108879
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108879