Bioactive Glass Nanoparticles-Loaded Poly(ɛ-caprolactone) Nanofiber as Substrate for ARPE-19 Cells
Joint Authors
Henrique Lima, Tadeu
Fernandes-Cunha, Gabriella Maria
Jensen, Carlos Eduardo de Matos
Zhao, Min
Behar-Cohen, Francine
Da Silva, Gisele Rodrigues
Silva-Cunha, Armando
Oréfice, Rodrigo Lambert
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-08
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Bioactive glass nanoparticles-loaded poly(ɛ-caprolactone) nanofibers (BIOG PCL nanofibers) were synthesized and evaluated as substrates for ocular cells (ARPE-19).
BIOG PCL nanofibers were characterized using SEM, FTIR, and DSC, and the in vitro degradation profile was also investigated.
The in vitro ocular biocompatibility of nanofibers was exploited in Müller glial cells (MIO-M1 cells) and in chorioallantoic membrane (CAM); and the proliferative capacity, cytotoxicity, and functionality were evaluated.
Finally, ARPE-19 cells were seeded onto BIOG PCL nanofibers and they were investigated as supports for in vitro cell adhesion and proliferation.
SEM images revealed the incorporation of BIOG nanoparticles into PCL nanofibers.
Nanoparticles did not induce modifications in the chemical structure and semicrystalline nature of PCL in the nanofiber, as shown by FTIR and DSC.
MIO-M1 cells exposed to BIOG PCL nanofibers showed viability, and they were able to proliferate and to express GFAP, indicating cellular functionality.
Moreover, nanofibers were well tolerated by CAM.
These findings suggested the in vitro ocular biocompatibility and absence of toxicity of these nanofibers.
Finally, the BIOG nanoparticles modulated the protein adsorption, and, subsequently, ARPE-19 cells adhered and proliferated onto the nanostructured supports, establishing cell-substrate interactions.
In conclusion, the biodegradable and biocompatible BIOG PCL nanofibers supported the ARPE-19 cells.
American Psychological Association (APA)
Henrique Lima, Tadeu& Fernandes-Cunha, Gabriella Maria& Jensen, Carlos Eduardo de Matos& Oréfice, Rodrigo Lambert& Silva-Cunha, Armando& Zhao, Min…[et al.]. 2016. Bioactive Glass Nanoparticles-Loaded Poly(ɛ-caprolactone) Nanofiber as Substrate for ARPE-19 Cells. Journal of Nanomaterials،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1109173
Modern Language Association (MLA)
Henrique Lima, Tadeu…[et al.]. Bioactive Glass Nanoparticles-Loaded Poly(ɛ-caprolactone) Nanofiber as Substrate for ARPE-19 Cells. Journal of Nanomaterials No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1109173
American Medical Association (AMA)
Henrique Lima, Tadeu& Fernandes-Cunha, Gabriella Maria& Jensen, Carlos Eduardo de Matos& Oréfice, Rodrigo Lambert& Silva-Cunha, Armando& Zhao, Min…[et al.]. Bioactive Glass Nanoparticles-Loaded Poly(ɛ-caprolactone) Nanofiber as Substrate for ARPE-19 Cells. Journal of Nanomaterials. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1109173
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1109173