Development and Characterization of an Electroless Plated SilverCysteine Sensor Platform for the Electrochemical Determination of Aflatoxin B1
Joint Authors
Wendiro, Deborah
Vuzi, Peter California
Wacoo, Alex Paul
Ocheng, Mathew
Hawumba, Joseph F.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
An electroless plated silver/cysteine sensor platform [Glass|silver|cysteine|aflatoxin B1|horseradish peroxidase] for the Electrochemical detection of aflatoxin B1 was developed and characterized.
This involved four major steps: (1) an electroless deposition of silver (plating) onto a glass slide, (2) immobilization of cysteine; (3) conjugation of aflatoxin B1 to cysteine groups; and (4) blocking of free cysteine groups with horseradish peroxidase (HRP).
The binding of cysteine to the silver was demonstrated by the disappearance of thiol (S-H) groups at 2500 cm−1 using Fourier transmittance infrared spectra (FT-IR), while the subsequent steps in the assembly of sensor platform were monitored using both FT-IR and cyclic voltammetry, respectively.
The sensor platform exhibited a broadened nonsymmetrical redox couple as indicated by cyclic voltammetry.
The platform was further characterized for sensitivity and limit of detection.
The indirect competitive immunoassay format, whereby free and immobilized aflatoxin B1 on the sensor competed for the binding site of free anti-aflatoxin B1 antibody, was used at various concentrations of aflatoxin B1.
The sensor generated differential staircase voltammogram that was inversely proportional to the concentration of aflatoxin B1 and aflatoxin B1 in the range of 0.06–1.1 ng/mL with a detection limit of 0.08 ng/mL could be detected.
American Psychological Association (APA)
Wacoo, Alex Paul& Ocheng, Mathew& Wendiro, Deborah& Vuzi, Peter California& Hawumba, Joseph F.. 2015. Development and Characterization of an Electroless Plated SilverCysteine Sensor Platform for the Electrochemical Determination of Aflatoxin B1. Journal of Sensors،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1110407
Modern Language Association (MLA)
Wacoo, Alex Paul…[et al.]. Development and Characterization of an Electroless Plated SilverCysteine Sensor Platform for the Electrochemical Determination of Aflatoxin B1. Journal of Sensors No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1110407
American Medical Association (AMA)
Wacoo, Alex Paul& Ocheng, Mathew& Wendiro, Deborah& Vuzi, Peter California& Hawumba, Joseph F.. Development and Characterization of an Electroless Plated SilverCysteine Sensor Platform for the Electrochemical Determination of Aflatoxin B1. Journal of Sensors. 2015. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1110407
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1110407