Bone Marrow Mesenchymal Stem Cells Inhibit Lipopolysaccharide-Induced Inflammatory Reactions in Macrophages and Endothelial Cells

Joint Authors

Li, Dequan
Wang, Cong
Chi, Chuang
Wang, Yuanyuan
Zhao, Jing
Fang, Jun
Pan, Jingye

Source

Mediators of Inflammation

Issue

Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2016-01-26

Country of Publication

Egypt

No. of Pages

9

Main Subjects

Diseases

Abstract EN

Background.

Systemic inflammatory response syndrome (SIRS) accompanied by trauma can lead to multiple organ dysfunction syndrome (MODS) and even death.

Early inhibition of the inflammation is necessary for damage control.

Bone marrow mesenchymal stem cells (BMSCs), as a novel therapy modality, have been shown to reduce inflammatory responses in human and animal models.

Methods.

In this study, we used Western blot, quantitative PCR, and enzyme-linked immunosorbent assay (ELISA) to assess the activity of BMSCs to suppress the inflammation induced by lipopolysaccharide (LPS) in human umbilical cord endothelial cells (HUVECs) and alveolar macrophages.

Results.

Our results demonstrated that LPS caused an inflammatory response in alveolar macrophages and HUVECs, increased permeability of HUVEC, upregulated expression of toll-like receptor (TLR) 2, TLR4, phosphorylated p65, downregulated release of IL10, and promoted release of TNF-α in both cells.

Coculture with BMSCs attenuated all of these activities induced by LPS in the two tested cell types.

Conclusions.

Together, our results demonstrate that BMSCs dosage dependently attenuates the inflammation damage of alveolar macrophages and HUVECs induced by LPS.

American Psychological Association (APA)

Li, Dequan& Wang, Cong& Chi, Chuang& Wang, Yuanyuan& Zhao, Jing& Fang, Jun…[et al.]. 2016. Bone Marrow Mesenchymal Stem Cells Inhibit Lipopolysaccharide-Induced Inflammatory Reactions in Macrophages and Endothelial Cells. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1110971

Modern Language Association (MLA)

Li, Dequan…[et al.]. Bone Marrow Mesenchymal Stem Cells Inhibit Lipopolysaccharide-Induced Inflammatory Reactions in Macrophages and Endothelial Cells. Mediators of Inflammation No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1110971

American Medical Association (AMA)

Li, Dequan& Wang, Cong& Chi, Chuang& Wang, Yuanyuan& Zhao, Jing& Fang, Jun…[et al.]. Bone Marrow Mesenchymal Stem Cells Inhibit Lipopolysaccharide-Induced Inflammatory Reactions in Macrophages and Endothelial Cells. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1110971

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1110971