Bone Marrow Mesenchymal Stem Cells Inhibit Lipopolysaccharide-Induced Inflammatory Reactions in Macrophages and Endothelial Cells
Joint Authors
Li, Dequan
Wang, Cong
Chi, Chuang
Wang, Yuanyuan
Zhao, Jing
Fang, Jun
Pan, Jingye
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Systemic inflammatory response syndrome (SIRS) accompanied by trauma can lead to multiple organ dysfunction syndrome (MODS) and even death.
Early inhibition of the inflammation is necessary for damage control.
Bone marrow mesenchymal stem cells (BMSCs), as a novel therapy modality, have been shown to reduce inflammatory responses in human and animal models.
Methods.
In this study, we used Western blot, quantitative PCR, and enzyme-linked immunosorbent assay (ELISA) to assess the activity of BMSCs to suppress the inflammation induced by lipopolysaccharide (LPS) in human umbilical cord endothelial cells (HUVECs) and alveolar macrophages.
Results.
Our results demonstrated that LPS caused an inflammatory response in alveolar macrophages and HUVECs, increased permeability of HUVEC, upregulated expression of toll-like receptor (TLR) 2, TLR4, phosphorylated p65, downregulated release of IL10, and promoted release of TNF-α in both cells.
Coculture with BMSCs attenuated all of these activities induced by LPS in the two tested cell types.
Conclusions.
Together, our results demonstrate that BMSCs dosage dependently attenuates the inflammation damage of alveolar macrophages and HUVECs induced by LPS.
American Psychological Association (APA)
Li, Dequan& Wang, Cong& Chi, Chuang& Wang, Yuanyuan& Zhao, Jing& Fang, Jun…[et al.]. 2016. Bone Marrow Mesenchymal Stem Cells Inhibit Lipopolysaccharide-Induced Inflammatory Reactions in Macrophages and Endothelial Cells. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1110971
Modern Language Association (MLA)
Li, Dequan…[et al.]. Bone Marrow Mesenchymal Stem Cells Inhibit Lipopolysaccharide-Induced Inflammatory Reactions in Macrophages and Endothelial Cells. Mediators of Inflammation No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1110971
American Medical Association (AMA)
Li, Dequan& Wang, Cong& Chi, Chuang& Wang, Yuanyuan& Zhao, Jing& Fang, Jun…[et al.]. Bone Marrow Mesenchymal Stem Cells Inhibit Lipopolysaccharide-Induced Inflammatory Reactions in Macrophages and Endothelial Cells. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1110971
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1110971