2,3,5,4′-Tetrahydroxystilbene-2-O-β-glucoside Isolated from Polygoni Multiflori Ameliorates the Development of Periodontitis

Abstract EN

Periodontitis, a chronic infection by periodontopathic bacteria, induces uncontrolled inflammation, which leads to periodontal tissue destruction.

2,3,5,4′-Tetrahydroxystilbene-2-O-beta-glucoside (THSG), a polyphenol extracted from Polygoni Multiflori, reportedly has anti-inflammatory properties.

In this study, we investigated the mechanisms of THSG on the Porphyromonas gingivalis-induced inflammatory responses in human gingival fibroblasts and animal modeling of ligature-induced periodontitis.

Human gingival fibroblast cells were treated with lipopolysaccharide (LPS) extracted from P.

gingivalis in the presence of resveratrol or THSG to analyze the expression of TNF-α, IL-1β, and IL-6 genes.

Increased AMP-activated protein kinase (AMPK) activation and SirT1 expression were induced by THSG.

Treatment of THSG decreased the expression of LPS-induced inflammatory cytokines, enhanced AMPK activation, and increased the expression of SirT1.

In addition, it suppressed the activation of NF-κB when cells were stimulated with P.

gingivalis LPS.

The anti-inflammatory effect of THSG and P.

Multiflori crude extracts was reproduced in ligature-induced periodontitis animal modeling.

In conclusion, THSG inhibited the inflammatory responses of P.

gingivalis-stimulated human gingival fibroblasts and ameliorated ligature-induced periodontitis in animal model.