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Autocrine Acetylcholine, Induced by IL-17A via NFκB and ERK12 Pathway Activation, Promotes MUC5AC and IL-8 Synthesis in Bronchial Epithelial Cells
Joint Authors
Bonanno, Anna
Riccobono, Loredana
Albano, Giusy Daniela
Di Sano, Caterina
Siena, Liboria
Gagliardo, Rosalia
Profita, Mirella
Montalbano, Angela Marina
Ferraro, Maria
Anzalone, Giulia
Pieper, Michael Paul
Gjomarkaj, Mark
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-19
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
IL-17A is overexpressed in the lung during acute neutrophilic inflammation.
Acetylcholine (ACh) increases IL-8 and Muc5AC production in airway epithelial cells.
We aimed to characterize the involvement of nonneuronal components of cholinergic system on IL-8 and Muc5AC production in bronchial epithelial cells stimulated with IL-17A.
Bronchial epithelial cells were stimulated with recombinant human IL-17A (rhIL-17A) to evaluate the ChAT expression, the ACh binding and production, the IL-8 release, and the Muc5AC production.
Furthermore, the effectiveness of PD098,059 (inhibitor of MAPKK activation), Bay11-7082 (inhibitor of IkBα phosphorylation), Hemicholinium-3 (HCh-3) (choline uptake blocker), and Tiotropium bromide (Spiriva®) (anticholinergic drug) was tested in our in vitro model.
We showed that rhIL-17A increased the expression of ChAT, the levels of ACh binding and production, and the IL-8 and Muc5AC production in stimulated bronchial epithelial cells compared with untreated cells.
The pretreatment of the cells with PD098,059 and Bay11-7082 decreased the ChAT expression and the ACh production/binding, while HCh-3 and Tiotropium decreased the IL-8 and Muc5AC synthesis in bronchial epithelial cells stimulated with rhIL-17A.
IL-17A is involved in the IL-8 and Muc5AC production promoting, via NFκB and ERK1/2 pathway activation, the synthesis of ChAT, and the related activity of autocrine ACh in bronchial epithelial cells.
American Psychological Association (APA)
Montalbano, Angela Marina& Albano, Giusy Daniela& Bonanno, Anna& Riccobono, Loredana& Di Sano, Caterina& Ferraro, Maria…[et al.]. 2016. Autocrine Acetylcholine, Induced by IL-17A via NFκB and ERK12 Pathway Activation, Promotes MUC5AC and IL-8 Synthesis in Bronchial Epithelial Cells. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1111282
Modern Language Association (MLA)
Montalbano, Angela Marina…[et al.]. Autocrine Acetylcholine, Induced by IL-17A via NFκB and ERK12 Pathway Activation, Promotes MUC5AC and IL-8 Synthesis in Bronchial Epithelial Cells. Mediators of Inflammation No. 2016 (2016), pp.1-16.
https://search.emarefa.net/detail/BIM-1111282
American Medical Association (AMA)
Montalbano, Angela Marina& Albano, Giusy Daniela& Bonanno, Anna& Riccobono, Loredana& Di Sano, Caterina& Ferraro, Maria…[et al.]. Autocrine Acetylcholine, Induced by IL-17A via NFκB and ERK12 Pathway Activation, Promotes MUC5AC and IL-8 Synthesis in Bronchial Epithelial Cells. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1111282
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1111282