Early Transcriptional Changes Induced by Wntβ-Catenin Signaling in Hippocampal Neurons
Joint Authors
Pérez-Palma, Eduardo
Andrade, Víctor
Caracci, Mario O.
Bustos, Bernabé I.
Villaman, Camilo
Medina, Matías A.
Ávila, Miguel E.
Ugarte, Giorgia D.
De Ferrari, Giancarlo V.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-27
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Wnt/β-catenin signaling modulates brain development and function and its deregulation underlies pathological changes occurring in neurodegenerative and neurodevelopmental disorders.
Since one of the main effects of Wnt/β-catenin signaling is the modulation of target genes, in the present work we examined global transcriptional changes induced by short-term Wnt3a treatment (4 h) in primary cultures of rat hippocampal neurons.
RNAseq experiments allowed the identification of 170 differentially expressed genes, including known Wnt/β-catenin target genes such as Notum, Axin2, and Lef1, as well as novel potential candidates Fam84a, Stk32a, and Itga9.
Main biological processes enriched with differentially expressed genes included neural precursor (GO:0061364, p-adjusted = 2.5 × 10−7), forebrain development (GO:0030900, p-adjusted = 7.3 × 10−7), and stem cell differentiation (GO:0048863 p-adjusted = 7.3 × 10−7).
Likewise, following activation of the signaling cascade, the expression of a significant number of genes with transcription factor activity (GO:0043565, p-adjusted = 4.1 × 10−6) was induced.
We also studied molecular networks enriched upon Wnt3a activation and detected three highly significant expression modules involved in glycerolipid metabolic process (GO:0046486, p-adjusted = 4.5 × 10−19), learning or memory (GO:0007611, p-adjusted = 4.0 × 10−5), and neurotransmitter secretion (GO:0007269, p-adjusted = 5.3 × 10−12).
Our results indicate that Wnt/β-catenin mediated transcription controls multiple biological processes related to neuronal structure and activity that are affected in synaptic dysfunction disorders.
American Psychological Association (APA)
Pérez-Palma, Eduardo& Andrade, Víctor& Caracci, Mario O.& Bustos, Bernabé I.& Villaman, Camilo& Medina, Matías A.…[et al.]. 2016. Early Transcriptional Changes Induced by Wntβ-Catenin Signaling in Hippocampal Neurons. Neural Plasticity،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1113153
Modern Language Association (MLA)
Pérez-Palma, Eduardo…[et al.]. Early Transcriptional Changes Induced by Wntβ-Catenin Signaling in Hippocampal Neurons. Neural Plasticity No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1113153
American Medical Association (AMA)
Pérez-Palma, Eduardo& Andrade, Víctor& Caracci, Mario O.& Bustos, Bernabé I.& Villaman, Camilo& Medina, Matías A.…[et al.]. Early Transcriptional Changes Induced by Wntβ-Catenin Signaling in Hippocampal Neurons. Neural Plasticity. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1113153
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113153