CLOCK Promotes Endothelial Damage by Inducing Autophagy through Reactive Oxygen Species
Joint Authors
Guo, Daqiao
Lin, Changpo
Shi, Zhenyu
Qian, Ruizhe
Liu, Jianjun
Fan, Longhua
Tang, Xiao
Li, Xu
Li, Xiaobo
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
A number of recent studies have implicated that autophagy was activated by reactive oxygen species (ROS).
Our previous report indicated that CLOCK increased the accumulation of ROS under hypoxic conditions.
In this study, we investigated the mechanisms by which CLOCK mediated endothelial damage, focusing on the involvement of oxidative damage and autophagy.
Overexpression of CLOCK in human umbilical vein endothelial cells (HUVECs) showed inhibition of cell proliferation and higher autophagosome with an increased expression of Beclin1 and LC3-I/II under hypoxic conditions.
In contrast, CLOCK silencing reversed these effects.
Interestingly, pretreatment with 3-methyladenine (3-MA) resulted in the attenuation of CLOCK-induced cell autophagy and but did not influence the production of intracellular reactive oxygen species (ROS).
Furthermore, Tiron (4,5-dihydroxy-1,3-benzene disulfonic acid-disodium salt), a ROS scavenger, significantly attenuated CLOCK-induced cell autophagy.
In addition, we found that overexpression of CLOCK had no significant effects on the production of ROS and expression of Beclin1 and LC3-I/II under normoxic conditions in HUVEC.
In this present investigation, our results suggested a novel mechanism of action of CLOCK in HUVECs, opening up the possibility of targeting CLOCK for the treatment of vascular diseases.
American Psychological Association (APA)
Tang, Xiao& Lin, Changpo& Guo, Daqiao& Qian, Ruizhe& Li, Xiaobo& Shi, Zhenyu…[et al.]. 2016. CLOCK Promotes Endothelial Damage by Inducing Autophagy through Reactive Oxygen Species. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1114789
Modern Language Association (MLA)
Tang, Xiao…[et al.]. CLOCK Promotes Endothelial Damage by Inducing Autophagy through Reactive Oxygen Species. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1114789
American Medical Association (AMA)
Tang, Xiao& Lin, Changpo& Guo, Daqiao& Qian, Ruizhe& Li, Xiaobo& Shi, Zhenyu…[et al.]. CLOCK Promotes Endothelial Damage by Inducing Autophagy through Reactive Oxygen Species. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1114789
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114789