Functionalized Fullerene Increases NF-κB Activity and Blocks Genotoxic Effect of Oxidative Stress in Serum-Starving Human Embryo Lung Diploid Fibroblasts

Abstract EN

The influence of a water-soluble [ 60 ] fullerene derivative containing five residues of 3-phenylpropionic acid and a chlorine addend appended to the carbon cage (F-828) on serum-starving human embryo lung diploid fibroblasts (HELFs) was studied.

Serum deprivation evokes oxidative stress in HELFs.

Cultivation of serum-starving HELFs in the presence of 0.1–1 µM F-828 significantly decreases the level of free radicals, inhibits autophagy, and represses expression of NOX4 and NRF2 proteins.

The activity of NF-κB substantially grows up in contrast to the suppressed NRF2 activity.

In the presence of 0.2–0.25 µM F-828, the DSB rate and apoptosis level dramatically decrease.

The maximum increase of proliferative activity of the HELFs and maximum activity of NF-κB are observed at these concentration values.

Conclusion.

Under the conditions of oxidative stress evoked by serum deprivation the water-soluble fullerene derivative F-828 used in concentrations of 0.1 to 1 µM strongly stimulates the NF-κB activity and represses the NRF2 activity in HELFs.