Transcriptome-Wide Analysis Reveals the Role of PPARγ Controlling the Lipid Metabolism in Goat Mammary Epithelial Cells
Joint Authors
Loor, Juan J.
Zhao, Wangsheng
Zhang, Changhui
Shahzad, Khuram
Shi, Hengbo
Luo, Jun
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-13
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
To explore the large-scale effect of peroxisome proliferator-activated receptor γ (PPARG) in goat mammary epithelial cells (GMEC), an oligonucleotide microarray platform was used for transcriptome profiling in cells overexpressing PPARG and incubated with or without rosiglitazone (ROSI, a PPARγ agonist).
A total of 1143 differentially expressed genes (DEG) due to treatment were detected.
The Dynamic Impact Approach (DIA) analysis uncovered the most impacted and induced pathways “fatty acid elongation in mitochondria,” “glycosaminoglycan biosynthesis-keratan sulfate,” and “pentose phosphate pathway.” The data highlights the central role of PPARG in milk fatty acid metabolism via controlling fatty acid elongation, biosynthesis of unsaturated fatty acid, lipid formation, and lipid secretion; furthermore, its role related to carbohydrate metabolism promotes the production of intermediates required for milk fat synthesis.
Analysis of upstream regulators indicated that PPARG participates in multiple physiological processes via controlling or cross talking with other key transcription factors such as PPARD and NR1H3 (also known as liver-X-receptor-α).
This transcriptome-wide analysis represents the first attempt to better understand the biological relevance of PPARG expression in ruminant mammary cells.
Overall, the data underscored the importance of PPARG in mammary lipid metabolism and transcription factor control.
American Psychological Association (APA)
Shi, Hengbo& Zhao, Wangsheng& Zhang, Changhui& Shahzad, Khuram& Luo, Jun& Loor, Juan J.. 2016. Transcriptome-Wide Analysis Reveals the Role of PPARγ Controlling the Lipid Metabolism in Goat Mammary Epithelial Cells. PPAR Research،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1115365
Modern Language Association (MLA)
Shi, Hengbo…[et al.]. Transcriptome-Wide Analysis Reveals the Role of PPARγ Controlling the Lipid Metabolism in Goat Mammary Epithelial Cells. PPAR Research No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1115365
American Medical Association (AMA)
Shi, Hengbo& Zhao, Wangsheng& Zhang, Changhui& Shahzad, Khuram& Luo, Jun& Loor, Juan J.. Transcriptome-Wide Analysis Reveals the Role of PPARγ Controlling the Lipid Metabolism in Goat Mammary Epithelial Cells. PPAR Research. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1115365
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1115365