Exogenous Heparan Sulfate Enhances the TGF-β3-Induced Chondrogenesis in Human Mesenchymal Stem Cells by Activating TGF-βSmad Signaling
Joint Authors
Chen, Juan
Wang, Yongqian
Chen, Chong
Lian, Chengjie
Zhou, Taifeng
Gao, Bo
Wu, Zizhao
Xu, Caixia
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-13
Country of Publication
Egypt
No. of Pages
10
Abstract EN
Heparan sulfate (HS) interacts with growth factors and has been implicated in regulating chondrogenesis.
However, the effect of HS on TGF-β-mediated mesenchymal stem cell (MSC) chondrogenesis and molecular mechanisms remains unknown.
In this study, we explored the effects of exogenous HS alone and in combination with TGF-β3 on chondrogenic differentiation of human MSCs and possible signal mechanisms.
The results indicated that HS alone had no obvious effects on chondrogenic differentiation of human MSCs and TGF-β/Smad2/3 signal pathways.
However, the combined TGF-β3/HS treatment resulted in a significant increase in GAG synthesis, cartilage matrix protein secretion, and cartilage-specific gene expression compared to cells treated with TGF-β3 alone.
Furthermore, HS inhibited type III TGF-β receptors (TβRIII) expression and increased TGF-β3-mediated ratio of the type II (TβRII) to the type I (TβRI) TGF-β receptors and phosphorylation levels of Smad2/3.
The inhibitor of the TGF-β/Smad signal, SB431542, not only completely inhibited HS-stimulated TGF-β3-mediated Smad2/3 phosphorylation but also completely inhibited the effects of HS on TGF-β3-induced chondrogenic differentiation.
These results demonstrate exogenous HS enhances TGF-β3-induced chondrogenic differentiation of human MSCs by activating TGF-β/Smad2/3 signaling.
American Psychological Association (APA)
Chen, Juan& Wang, Yongqian& Chen, Chong& Lian, Chengjie& Zhou, Taifeng& Gao, Bo…[et al.]. 2015. Exogenous Heparan Sulfate Enhances the TGF-β3-Induced Chondrogenesis in Human Mesenchymal Stem Cells by Activating TGF-βSmad Signaling. Stem Cells International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1116062
Modern Language Association (MLA)
Chen, Juan…[et al.]. Exogenous Heparan Sulfate Enhances the TGF-β3-Induced Chondrogenesis in Human Mesenchymal Stem Cells by Activating TGF-βSmad Signaling. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1116062
American Medical Association (AMA)
Chen, Juan& Wang, Yongqian& Chen, Chong& Lian, Chengjie& Zhou, Taifeng& Gao, Bo…[et al.]. Exogenous Heparan Sulfate Enhances the TGF-β3-Induced Chondrogenesis in Human Mesenchymal Stem Cells by Activating TGF-βSmad Signaling. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1116062
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116062