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Wharton’s Jelly-Derived Mesenchymal Stromal Cells and Fibroblast-Derived Extracellular Matrix Synergistically Activate Apoptosis in a p21-Dependent Mechanism in WHCO1 and MDA MB 231 Cancer Cells In Vitro
Joint Authors
Pepper, Michael S.
Dzobo, Kevin
Vogelsang, Matjaz
Thomford, Nicholas E.
Dandara, Collet
Kallmeyer, Karlien
Parker, M. Iqbal
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-10
Country of Publication
Egypt
No. of Pages
17
Abstract EN
The tumour microenvironment plays a crucial role in tumour progression and comprises tumour stroma which is made up of different cell types and the extracellular matrix (ECM).
Mesenchymal stromal cells (MSCs) are part of the tumour stroma and may have conflicting effects on tumour growth.
In this study we investigated the effect of Wharton’s Jelly-derived MSCs (WJ-MSCs) and a fibroblast-derived ECM (fd-ECM) on esophageal (WHCO1) and breast (MDA MB 231) cancer cells in vitro.
Both WJ-MSCs and the fd-ECM, alone or in combination, downregulate PCNA, cyclin D1, Bcl-2, Bcl-xL, and MMPs and upregulate p53 and p21.
p21 induction resulted in G2 phase cell cycle arrest and induced apoptosis in vitro.
Our data suggest that p21 induction is via p53-dependent and p53-independent mechanisms in WHCO1 and MDA MB 231 cells, respectively.
Vascular endothelial growth factor, Akt, and Nodal pathways were downregulated in cancer cells cocultured with WJ-MSCs.
We also demonstrate that WJ-MSCs effects on cancer cells appear to be short-lived whilst the fd-ECM effect is long-lived.
This study shows the influence of tumour microenvironment on cancer cell behaviour and provides alternative therapeutic targets for potential regulation of tumour cells.
American Psychological Association (APA)
Dzobo, Kevin& Vogelsang, Matjaz& Thomford, Nicholas E.& Dandara, Collet& Kallmeyer, Karlien& Pepper, Michael S.…[et al.]. 2016. Wharton’s Jelly-Derived Mesenchymal Stromal Cells and Fibroblast-Derived Extracellular Matrix Synergistically Activate Apoptosis in a p21-Dependent Mechanism in WHCO1 and MDA MB 231 Cancer Cells In Vitro. Stem Cells International،Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1116659
Modern Language Association (MLA)
Dzobo, Kevin…[et al.]. Wharton’s Jelly-Derived Mesenchymal Stromal Cells and Fibroblast-Derived Extracellular Matrix Synergistically Activate Apoptosis in a p21-Dependent Mechanism in WHCO1 and MDA MB 231 Cancer Cells In Vitro. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-17.
https://search.emarefa.net/detail/BIM-1116659
American Medical Association (AMA)
Dzobo, Kevin& Vogelsang, Matjaz& Thomford, Nicholas E.& Dandara, Collet& Kallmeyer, Karlien& Pepper, Michael S.…[et al.]. Wharton’s Jelly-Derived Mesenchymal Stromal Cells and Fibroblast-Derived Extracellular Matrix Synergistically Activate Apoptosis in a p21-Dependent Mechanism in WHCO1 and MDA MB 231 Cancer Cells In Vitro. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1116659
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116659