Spheroid Culture of Mesenchymal Stem Cells

Joint Authors

Cesarz, Zoe
Tamama, Kenichi

Source

Stem Cells International

Issue

Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-11, 11 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2015-11-16

Country of Publication

Egypt

No. of Pages

11

Abstract EN

Compared with traditional 2D adherent cell culture, 3D spheroidal cell aggregates, or spheroids, are regarded as more physiological, and this technique has been exploited in the field of oncology, stem cell biology, and tissue engineering.

Mesenchymal stem cells (MSCs) cultured in spheroids have enhanced anti-inflammatory, angiogenic, and tissue reparative/regenerative effects with improved cell survival after transplantation.

Cytoskeletal reorganization and drastic changes in cell morphology in MSC spheroids indicate a major difference in mechanophysical properties compared with 2D culture.

Enhanced multidifferentiation potential, upregulated expression of pluripotency marker genes, and delayed replicative senescence indicate enhanced stemness in MSC spheroids.

Furthermore, spheroid formation causes drastic changes in the gene expression profile of MSC in microarray analyses.

In spite of these significant changes, underlying molecular mechanisms and signaling pathways triggering and sustaining these changes are largely unknown.

American Psychological Association (APA)

Cesarz, Zoe& Tamama, Kenichi. 2015. Spheroid Culture of Mesenchymal Stem Cells. Stem Cells International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117384

Modern Language Association (MLA)

Cesarz, Zoe& Tamama, Kenichi. Spheroid Culture of Mesenchymal Stem Cells. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1117384

American Medical Association (AMA)

Cesarz, Zoe& Tamama, Kenichi. Spheroid Culture of Mesenchymal Stem Cells. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117384

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1117384