Recombinant α-Klotho Protein Alleviated Acute Cardiorenal Injury in a Mouse Model of Lipopolysaccharide-Induced Septic Cardiorenal Syndrome Type 5
Joint Authors
Liu, Xi
Niu, Yangyang
Zhang, Xiaoqin
Zhang, Yingying
Yu, Ying
Huang, Jieli
Li, Jiangtao
Yu, Chen
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-11
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Background and Aims.
Klotho is an aging-suppressor gene mainly expressed in the renal tubules.
The klotho gene encodes the α-klotho protein, which has many functions.
Previous studies have found that α-klotho protein has a cardiorenal protective function.
α-Klotho deficiency renders the kidney more susceptible to injury and results in cardiovascular calcification and left ventricular hypertrophy in chronic kidney disease.
However, the role of α-klotho in acute heart injury and acute kidney injury with sepsis remains unknown.
This study aimed to investigate the effects and mechanisms of α-klotho in septic cardiorenal injury.
Methods.
Male 8-week-old C57BL/6 mice were randomly assigned to the control group, lipopolysaccharide (LPS; 10 mg/kg) group, LPS (10 mg/kg)+α-klotho (0.01 mg/kg) group, and LPS (10 mg/kg)+α-klotho (0.02 mg/kg) group.
Recombinant α-klotho was intraperitoneally injected an hour before LPS injection.
Mice were euthanized at 24 h after LPS injection.
The serum troponin, brain natriuretic peptide (BNP), neutrophil gelatinase-associated lipocalin (NGAL), and creatinine levels were measured in all groups at 24 h.
Biomarkers of mice heart apoptosis, inflammation, oxidative stress, and endoplasmic reticulum stress, such as caspase-3, interleukin 1 (IL-1), reactive oxygen species (ROS), and glucose-regulated protein 78 (GRP78), were also measured.
Results.
α-Klotho was mainly expressed in mice kidneys and was undetectable in the control mice hearts.
α-Klotho substantially decreased after LPS injection.
In the LPS group, the serum troponin levels significantly increased as early as 6 h (p<0.05) after LPS injection, while the BNP, NGAL, and creatinine levels significantly increased at 24 h (p<0.05).
Pretreatment with α-klotho significantly ameliorated acute cardiorenal injury.
In the LPS+α-klotho (0.01 mg/kg) group, the levels of apoptosis, inflammation, and oxidative stress were decreased, while the level of endoplasmic reticulum stress was elevated.
Conclusions.
α-Klotho significantly alleviates acute cardiorenal injury in LPS-induced septic cardiorenal injury due to the inhibition of apoptosis, inflammation, and oxidation, as well as the regulation of endoplasmic reticulum stress levels.
American Psychological Association (APA)
Liu, Xi& Niu, Yangyang& Zhang, Xiaoqin& Zhang, Yingying& Yu, Ying& Huang, Jieli…[et al.]. 2019. Recombinant α-Klotho Protein Alleviated Acute Cardiorenal Injury in a Mouse Model of Lipopolysaccharide-Induced Septic Cardiorenal Syndrome Type 5. Analytical Cellular Pathology،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1117858
Modern Language Association (MLA)
Liu, Xi…[et al.]. Recombinant α-Klotho Protein Alleviated Acute Cardiorenal Injury in a Mouse Model of Lipopolysaccharide-Induced Septic Cardiorenal Syndrome Type 5. Analytical Cellular Pathology No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1117858
American Medical Association (AMA)
Liu, Xi& Niu, Yangyang& Zhang, Xiaoqin& Zhang, Yingying& Yu, Ying& Huang, Jieli…[et al.]. Recombinant α-Klotho Protein Alleviated Acute Cardiorenal Injury in a Mouse Model of Lipopolysaccharide-Induced Septic Cardiorenal Syndrome Type 5. Analytical Cellular Pathology. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1117858
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117858