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A Combination of UTMD-Mediated HIF-1α shRNA Transfection and TAE in the Treatment of Hepatic Cancer
Joint Authors
Liao, Yangying
Luo, Haibo
He, Zhizhong
Kuang, Yongpei
Chen, Peifen
Zhang, Xiuzhen
Chen, Junjun
Wen, Qirong
Xie, Yuhuan
Ding, Shangwei
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-19
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
To explore the antitumor effect of hypoxia-inducible factor-1α short hairpin RNA (HIF-1α shRNA) delivered by ultrasound targeted microbubble destruction (UTMD) and transcatheter arterial embolization (TAE) on rats with hepatic cancer.
After the models of transplantation hepatoma were established, Wistar rats were randomly divided into 4 groups: Control group, UTMD group, TAE group, and UTMD+TAE group.
Contrast-enhanced ultrasound (CEUS) was used to monitor tumor size on day 14 after four different treatments.
Western blotting and immunohistochemistry were applied to measure the protein level of HIF-1α and VEGF in the hepatic cancer tissue.
In comparison with UTMD+TAE group (21.25±10.68 days), the mean survival time was noticeably shorter in the Control group and TAE group (13.02±4.30 days and 15.03±7.32 days) (p<0.05, respectively).
There was no statistical difference between UTMD+TAE group and UTMD group of the mean survival time (p>0.05).
In addition, our results proved that the tumor sizes in UTMD+TAE group were obviously smaller than those in other groups (p<0.05, respectively).
By CEUS, we clearly found that the tumor size was the smallest on day 14 in the UTMD+TAE group.
The western blotting and immunohistochemistry results proved that the protein levels of HIF-1α and VEGF in UTMD+TAE group were obviously lower than those in TAE group and Control group on days 7 and 14 (p<0.05, respectively).
However, there was no statistical difference between UTMD+TAE group and UTMD group (p>0.05).
In this study we tried to explore the antitumor effect through a combination of UTMD-mediated HIF-1α shRNA transfection and TAE on rats with hepatic cancer.
Our results showed that UTMD-mediated HIF-1α shRNA transfection and TAE can obviously silence HIF-1α and VEGF expression, thereby successfully inhibiting the growth of the tumor.
American Psychological Association (APA)
Liao, Yangying& Luo, Haibo& He, Zhizhong& Kuang, Yongpei& Chen, Peifen& Zhang, Xiuzhen…[et al.]. 2019. A Combination of UTMD-Mediated HIF-1α shRNA Transfection and TAE in the Treatment of Hepatic Cancer. BioMed Research International،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1123581
Modern Language Association (MLA)
Liao, Yangying…[et al.]. A Combination of UTMD-Mediated HIF-1α shRNA Transfection and TAE in the Treatment of Hepatic Cancer. BioMed Research International No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1123581
American Medical Association (AMA)
Liao, Yangying& Luo, Haibo& He, Zhizhong& Kuang, Yongpei& Chen, Peifen& Zhang, Xiuzhen…[et al.]. A Combination of UTMD-Mediated HIF-1α shRNA Transfection and TAE in the Treatment of Hepatic Cancer. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1123581
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1123581