Effects of Norspermidine on Dual‐Species Biofilms Composed of Streptococcus mutans and Streptococcus sanguinis
Joint Authors
Li, Mingyun
Huang, Shengbin
Sun, Yan
Pan, Yihuai
Sun, Yu
Qiu, Wei
Tu, Huanxin
Zhang, Keke
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-03
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The present study aimed at investigating the influence of norspermidine on the formation of dual-species biofilms composed of Streptococcus mutans (S.
mutans) and Streptococcus sanguinis (S.
sanguinis).
Crystal violet assay was conducted to assess the formation of single-species biofilms of S.
mutans and S.
sanguinis, and the growth curve was carefully observed to monitor the growth of these two species of bacteria.
Fluorescence in situ hybridization (FISH) and MTT array were used to analyze the composition and metabolic activity of the dual-species biofilms, respectively.
Extracellular polysaccharides (EPS)/bacteria staining, anthrone method, and scanning electron microscopy (SEM) imaging were conducted to study the synthesis of EPS by dual-species biofilms.
Lactic acid assay and pH were measured to detect dual-species biofilm acid production.
We found that norspermidine had different effects on S.
mutans and S.
sanguinis including their growth and biofilm formation.
Norspermidine regulated the composition of the dual-species biofilms, decreased the ratio of S.
mutans in dual-species biofilms, and reduced the metabolic activity, EPS synthesis, and acid production of dual-species biofilms.
Norspermidine regulated dual-species biofilms in an ecological way, suggesting that it may be a potent reagent for controlling dental biofilms and managing dental caries.
American Psychological Association (APA)
Sun, Yan& Pan, Yihuai& Sun, Yu& Li, Mingyun& Huang, Shengbin& Qiu, Wei…[et al.]. 2019. Effects of Norspermidine on Dual‐Species Biofilms Composed of Streptococcus mutans and Streptococcus sanguinis. BioMed Research International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1123588
Modern Language Association (MLA)
Sun, Yan…[et al.]. Effects of Norspermidine on Dual‐Species Biofilms Composed of Streptococcus mutans and Streptococcus sanguinis. BioMed Research International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1123588
American Medical Association (AMA)
Sun, Yan& Pan, Yihuai& Sun, Yu& Li, Mingyun& Huang, Shengbin& Qiu, Wei…[et al.]. Effects of Norspermidine on Dual‐Species Biofilms Composed of Streptococcus mutans and Streptococcus sanguinis. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1123588
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1123588