Dihydromyricetin Attenuates TNF-α-Induced Endothelial Dysfunction through miR-21-Mediated DDAH1ADMANO Signal Pathway
Joint Authors
Jiang, Pei
Dang, Rui-Li
Zhang, Bi-Kui
Yang, Dafeng
Tan, Shenglan
Yang, Zhousheng
Qin, Caie
Yuan, Qiong
Yao, Xiaoxia
Qu, Jian
Lu, Qiong
Xu, Ping
Xiang, Daxiong
Chen, Lei
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-28
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Accumulating studies demonstrate that dihydromyricetin (DMY), a compound extracted from Chinese traditional herb, Ampelopsis grossedentata, attenuates atherosclerotic process by improvement of endothelial dysfunction.
However, the underlying mechanism remains poorly understood.
Thus, the aim of this study is to investigate the potential mechanism behind the attenuating effects of DMY on tumor necrosis factor alpha- (TNF-α-) induced endothelial dysfunction.
In response to TNF-α, microRNA-21 (miR-21) expression was significantly increased in human umbilical vein endothelial cells (HUVECs), in line with impaired endothelial dysfunction as evidenced by decreased tube formation and migration, endothelial nitric oxide synthase (eNOS) (ser1177) phosphorylation, dimethylarginine dimethylaminohydrolases 1 (DDAH1) expression and metabolic activity, and nitric oxide (NO) concentration as well as increased asymmetric dimethylarginine (ADMA) levels.
In contrast, DMY or blockade of miR-21 expression ameliorated endothelial dysfunction in HUVECs treated with TNF-α through downregulation of miR-21 expression, whereas these effects were abolished by overexpression of miR-21.
In addition, using a nonspecific NOS inhibitor, L-NAME, also abrogated the attenuating effects of DMY on endothelial dysfunction.
Taken together, these data demonstrated that miR-21-mediated DDAH1/ADMA/NO signal pathway plays an important role in TNF-α-induced endothelial dysfunction, and DMY attenuated endothelial dysfunction induced by TNF-α in a miR-21-dependent manner.
American Psychological Association (APA)
Yang, Dafeng& Tan, Shenglan& Yang, Zhousheng& Jiang, Pei& Qin, Caie& Yuan, Qiong…[et al.]. 2018. Dihydromyricetin Attenuates TNF-α-Induced Endothelial Dysfunction through miR-21-Mediated DDAH1ADMANO Signal Pathway. BioMed Research International،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1124116
Modern Language Association (MLA)
Yang, Dafeng…[et al.]. Dihydromyricetin Attenuates TNF-α-Induced Endothelial Dysfunction through miR-21-Mediated DDAH1ADMANO Signal Pathway. BioMed Research International No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1124116
American Medical Association (AMA)
Yang, Dafeng& Tan, Shenglan& Yang, Zhousheng& Jiang, Pei& Qin, Caie& Yuan, Qiong…[et al.]. Dihydromyricetin Attenuates TNF-α-Induced Endothelial Dysfunction through miR-21-Mediated DDAH1ADMANO Signal Pathway. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1124116
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124116