Melatonin Attenuates β-Glycerophosphate-Induced Calcification of Vascular Smooth Muscle Cells via a Wnt1β-Catenin Signaling Pathway
Joint Authors
Zhou, Yujie
Chen, Wei Ren
Yang, Jia Qi
Liu, Fang
Zhao, Ying Xin
Sha, Yuan
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Melatonin has been demonstrated to protect against calcification in cyclosporine nephrotoxicity.
The wingless-type MMTV integration site family member 1 (Wnt1)/β-catenin pathway is associated with cardiovascular calcification.
This study aimed to explore whether melatonin could attenuate VSMC calcification through regulating the Wnt1/β-catenin signaling pathway.
Methods.
The effects of melatonin on vascular calcification were investigated in vascular smooth muscle cells (VSMCs).
Calcium deposits were visualized by Alizarin Red Staining.
Calcium content and alkaline phosphatase (ALP) activity were used to evaluate osteogenic differentiation.
Western blots were used to measure the expression of runt-related transcription factor 2 (Runx2), α-smooth muscle actin (α-SMA), and cleaved caspase-3.
Results.
Melatonin markedly ameliorated calcium deposition and ALP activity.
Runx2 and cleaved caspase-3 were found to be reduced and α-SMA was found to be increased by melatonin, together with a decrease in apoptosis.
Immunofluorescence assay revealed a lower Runx2 protein level in the melatonin group.
Melatonin treatment significantly decreased the expression of Wnt1 and β-catenin.
Treatment with lithium chloride or transglutaminase 2 abrogated the protective effects of melatonin.
Conclusion.
Melatonin can attenuate β-GP-induced VSMC calcification through the suppression of Wnt1/β-catenin system.
American Psychological Association (APA)
Chen, Wei Ren& Zhou, Yujie& Yang, Jia Qi& Liu, Fang& Zhao, Ying Xin& Sha, Yuan. 2019. Melatonin Attenuates β-Glycerophosphate-Induced Calcification of Vascular Smooth Muscle Cells via a Wnt1β-Catenin Signaling Pathway. BioMed Research International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1124394
Modern Language Association (MLA)
Chen, Wei Ren…[et al.]. Melatonin Attenuates β-Glycerophosphate-Induced Calcification of Vascular Smooth Muscle Cells via a Wnt1β-Catenin Signaling Pathway. BioMed Research International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1124394
American Medical Association (AMA)
Chen, Wei Ren& Zhou, Yujie& Yang, Jia Qi& Liu, Fang& Zhao, Ying Xin& Sha, Yuan. Melatonin Attenuates β-Glycerophosphate-Induced Calcification of Vascular Smooth Muscle Cells via a Wnt1β-Catenin Signaling Pathway. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1124394
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124394