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Different Methylation of CpG-SNPs in Behcet’s Disease
Joint Authors
Yang, Peizeng
Huang, Yang
Tan, Handan
Cao, Qingfeng
Yuan, Gangxiang
Su, Guannan
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-16
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Purpose.
We recently performed an Epigenome-Wide Association Studies (EWAS) study in Behcet’s disease (BD) and identified various cytosine–phosphate–guanine (CpG) loci that were aberrantly methylated.
In the current study, we wanted to investigate whether these sites contained genetic polymorphisms and whether the frequency of these polymorphisms was altered in BD.
Methods.
A two-stage study was performed.
The first stage involved 358 BD patients and 704 healthy controls to investigate genetic variants of 10 CpG-SNPs (rs10454134, rs176249, rs3808620, rs10176517, rs11247118, rs78016579, rs9461624, rs10492166, rs34929465, and rs6507921) using an iPLEX Gold genotyping assay and a Sequenom MassARRAY.
In the second stage, an additional 172 independent BD patients and 330 healthy individuals are to confirm trends found in the first stage.
Results.
A higher frequency of both the rs10454134 AG genotypes (p = 0.008, OR = 1.413, 95% CI = 1.094-1.826) and a lower GG genotype frequency (p = 0.003, OR = 0.630, 95% CI = 0.465-0.854) were found in BD patients compared to the controls in the first stage.
However, after correcting for multiple comparisons, all associations identified in the first stage lost statistical significance.
The frequencies of the other CpG-SNPs investigated were not different between BD patients and controls.
The second stage was designed using an additional cohort to confirm the association with CpG-SNP, rs10454134.
The data failed to confirm the association between this CpG-SNP and BD.
Conclusions.
This study did not show an association between BD and CpG-SNPs in gene sites that were earlier shown to be aberrantly methylated.
American Psychological Association (APA)
Huang, Yang& Tan, Handan& Cao, Qingfeng& Yuan, Gangxiang& Su, Guannan& Yang, Peizeng. 2019. Different Methylation of CpG-SNPs in Behcet’s Disease. BioMed Research International،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1124611
Modern Language Association (MLA)
Huang, Yang…[et al.]. Different Methylation of CpG-SNPs in Behcet’s Disease. BioMed Research International No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1124611
American Medical Association (AMA)
Huang, Yang& Tan, Handan& Cao, Qingfeng& Yuan, Gangxiang& Su, Guannan& Yang, Peizeng. Different Methylation of CpG-SNPs in Behcet’s Disease. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1124611
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124611