Evaluation of Lsa46 and Lsa77 Leptospiral Proteins for Their Immunoprotective Activities in Hamster Model of Leptospirosis
Joint Authors
Heinemann, Marcos Bryan
Vasconcellos, Silvio A.
Teixeira, Aline F.
Fernandes, Luis G. V.
Souza Filho, Antonio
Souza, Gisele O.
Nascimento, Ana L. T. O.
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-10
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Leptospirosis is a neglected tropical disease caused by pathogenic Leptospira spp.
The lack of an effective vaccine favors the increase of the disease.
Currently, surface-exposed proteins are the main targets for the search of vaccine candidates.
In this study, we examined whether the surface Lsa46 and Lsa77 proteins, previously identified as laminin and plasminogen binding proteins, have the capacity of inducing protection and sterilizing immunity against challenge with virulent Leptospira in hamster model.
Animals were subcutaneously immunized with Lsa46, Lsa77, or a combination of both in Alum adjuvant and challenged intraperitoneally with L.
interrogans serovar Kennewicki strain Pomona Fromm.
Hamster immunization with Lsa46 or Lsa77 or both promoted a strong IgG response.
Th2- and Th1-biased immune responses were observed when Lsa46 and Lsa77 were individually administered, respectively, as detected by the IgG1/IgG2/3 ratio.
Immunized hamsters with the combined proteins induced a Th1-biased immune response.
Although the immunization with Lsa46 and Lsa77 stimulated protective immunity with reduction of bacterial burden, when compared to animals individually immunized with the proteins, the data was not statistically significant.
Thus, although promising, more studies are needed before the role of these proteins in stimulating sterilizing immunity in mammals is conclusively determined.
American Psychological Association (APA)
Teixeira, Aline F.& Fernandes, Luis G. V.& Souza Filho, Antonio& Souza, Gisele O.& Vasconcellos, Silvio A.& Heinemann, Marcos Bryan…[et al.]. 2018. Evaluation of Lsa46 and Lsa77 Leptospiral Proteins for Their Immunoprotective Activities in Hamster Model of Leptospirosis. BioMed Research International،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1124661
Modern Language Association (MLA)
Teixeira, Aline F.…[et al.]. Evaluation of Lsa46 and Lsa77 Leptospiral Proteins for Their Immunoprotective Activities in Hamster Model of Leptospirosis. BioMed Research International No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1124661
American Medical Association (AMA)
Teixeira, Aline F.& Fernandes, Luis G. V.& Souza Filho, Antonio& Souza, Gisele O.& Vasconcellos, Silvio A.& Heinemann, Marcos Bryan…[et al.]. Evaluation of Lsa46 and Lsa77 Leptospiral Proteins for Their Immunoprotective Activities in Hamster Model of Leptospirosis. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1124661
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124661