A Single Codon Optimization Enhances Recombinant Human TNF-α Vaccine Expression in Escherichia coli
Joint Authors
Zhang, Wei
Li, Wei-na
Zhang, Ying-qi
Wang, Zeng-lu
Xue, Xiao-Chang
Zhao, Ning
Wan, Yi
Chu, Chu
Zhang, Wangqian
Li, Jialin
Duan, Ruyi
Yu, Pei
Zhang, Kuo
Wang, Shuning
Hao, Qiang
Zhang, Cun
Li, Meng
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
As a proinflammatory cytokine, tumor necrosis factor-alpha (TNF-α) plays a pivotal role in various autoimmune diseases such as rheumatoid arthritis (RA).
Thus, TNF-α has been defined as a therapeutic target for RA.
Although some TNF-α antagonists including neutralizing monoclonal antibodies and soluble receptors have been approved to be successful in attenuating symptoms in patients suffering from RA, the long-term use of these passive immunization reagents could cause some problems like a variable degree of immunogenicity.
In the present study, in order to wake up active immune responses of RA patients, we developed a recombinant TNF-α therapeutic vaccine (named mrTNF-PADRE) by coupling a 12-amino acid universal Pan HLA-DR Epitope (PADRE) to the protein.
Codon optimization was performed to improve the secondary structure of mrTNF-PADRE mRNA to ensure its heterologous expression.
As a result, a single codon synonymous mutation greatly elevated recombinant protein expression (about 30% of the total bacteria proteins) in E.
coli as compared with the undetectable expression of the unoptimized gene.
Although expressed as insoluble inclusion bodies (IBs), the vaccine can be effectively prepared with a purity of over 95% by IBs washing and one-step gel-infiltration chromatography.
By this strategy, a stable yield of 5.2 mg purified mrTNF-PADRE per gram of cell paste could be obtained.
American Psychological Association (APA)
Chu, Chu& Zhang, Wangqian& Li, Jialin& Wan, Yi& Wang, Zeng-lu& Duan, Ruyi…[et al.]. 2018. A Single Codon Optimization Enhances Recombinant Human TNF-α Vaccine Expression in Escherichia coli. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1125563
Modern Language Association (MLA)
Chu, Chu…[et al.]. A Single Codon Optimization Enhances Recombinant Human TNF-α Vaccine Expression in Escherichia coli. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1125563
American Medical Association (AMA)
Chu, Chu& Zhang, Wangqian& Li, Jialin& Wan, Yi& Wang, Zeng-lu& Duan, Ruyi…[et al.]. A Single Codon Optimization Enhances Recombinant Human TNF-α Vaccine Expression in Escherichia coli. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1125563
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125563