Hydrogen-Rich Saline Activated Autophagy via HIF-1α Pathways in Neuropathic Pain Model
Joint Authors
Xie, Keliang
Wang, Huixing
Huo, Xiaodong
Chen, Hongguang
Li, Bo
Liu, Jingzhi
Ma, Wenting
Wang, Xiaojuan
Yu, Yonghao
Shi, Kemei
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-17
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Background.
Neuropathic pain is a chronic and intractable pain, with very few effective analgesics.
It involves an impaired cell autophagy process.
Hydrogen-rich saline (HRS) reportedly reduces allodynia and hyperalgesia in a neuropathic pain model; however, it is unknown whether these effects involve autophagy induction.
Methods.
We investigated the relationship between HRS and cell autophagy in a neuropathic pain model generated by chronic constriction injury (CCI) in Sprague–Dawley rats.
Rats received an intraperitoneal injection of HRS (10 mL/kg daily, from 1 day before until 14 days after CCI), 3MA (autophagy inhibitor), 2ME2 (HIF-1α inhibitor), or EDHB (HIF-1α agonist).
The mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were tested 1 day before and 1, 3, 7, 10, and 14 days after the operation.
HIF-1α and cell autophagy markers in the spinal cord were evaluated by western blotting and real-time PCR assays at 14 days after CCI.
Autophagosomes with double membranes were identified by transmission electron microscopy.
Results.
CCI caused behavioral hypersensitivity to mechanical and thermal stimulation in the hind-paw of the injured side.
HRS improved MWT and TWL, activated autophagy, and increased autophagosomes and autolysosomes in CCI rats.
3-MA aggravated hyperalgesia and allodynia and suppressed autophagy, while EDHB attenuated hyperalgesia and activated the autophagy procedure and the HIF-1α downstream target gene BNIP3.
HIF-1α inhibitors reversed the regulatory effects of HRS on autophagy in CCI rats at 14 days after spinal cord injury.
Conclusion.
HRS reduced mechanical hyperalgesia and activation of cell autophagy in neuropathic pain through a HIF1-dependent pathway.
American Psychological Association (APA)
Wang, Huixing& Huo, Xiaodong& Chen, Hongguang& Li, Bo& Liu, Jingzhi& Ma, Wenting…[et al.]. 2018. Hydrogen-Rich Saline Activated Autophagy via HIF-1α Pathways in Neuropathic Pain Model. BioMed Research International،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1126839
Modern Language Association (MLA)
Wang, Huixing…[et al.]. Hydrogen-Rich Saline Activated Autophagy via HIF-1α Pathways in Neuropathic Pain Model. BioMed Research International No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1126839
American Medical Association (AMA)
Wang, Huixing& Huo, Xiaodong& Chen, Hongguang& Li, Bo& Liu, Jingzhi& Ma, Wenting…[et al.]. Hydrogen-Rich Saline Activated Autophagy via HIF-1α Pathways in Neuropathic Pain Model. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1126839
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126839