Proteomic Analysis of Liver from Human Lipoprotein(a) Transgenic Mice Shows an Oxidative Stress and Lipid Export Response
Joint Authors
Rodger, Euan J.
Porteous, Carolyn M.
Jones, Gregory T.
Legge, Michael
Kleffmann, Torsten
McCormick, Sally P. A.
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-25
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
Mouse models of hypercholesterolaemia have been used to identify arterial proteins involved in atherosclerosis.
As the liver is extremely sensitive to dyslipidemia, one might expect major changes in the abundance of liver proteins in these models even before atherosclerosis develops.
Methods.
Lipid levels were measured and a proteomic approach was used to quantify proteins in the livers of mice with an elevated low-density lipoprotein (LDL) and the presence of lipoprotein(a) [Lp(a)] but no atherosclerosis.
Results.
The livers of Lp(a) mice showed an increased triglyceride but reduced phospholipid and oxidised lipid content.
Two-dimensional gel electrophoresis and mass spectrometry analysis identified 24 liver proteins with significantly increased abundance in Lp(a) mice (P<0.05).
A bioinformatic analysis of the 24 proteins showed the major effect was that of an enhanced antioxidant and lipid efflux response with significant increases in antioxidant (Park7, Gpx1, Prdx6, and Sod1) and lipid metabolism proteins (Fabp4, Acaa2, apoA4, and ApoA1).
Interestingly, human liver cells treated with Lp(a) showed significant increases in Gpx1 and Prdx6 but not Sod1 or Park7.
Conclusions.
The presence of human LDL and Lp(a) in mice promotes an enhanced flux of lipids into the liver which elicits an antioxidant and lipid export response before the onset of atherosclerosis.
The antioxidant response can be reproduced in human liver cells treated with Lp(a).
American Psychological Association (APA)
Rodger, Euan J.& Porteous, Carolyn M.& Jones, Gregory T.& Legge, Michael& Kleffmann, Torsten& McCormick, Sally P. A.. 2018. Proteomic Analysis of Liver from Human Lipoprotein(a) Transgenic Mice Shows an Oxidative Stress and Lipid Export Response. BioMed Research International،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1127016
Modern Language Association (MLA)
Rodger, Euan J.…[et al.]. Proteomic Analysis of Liver from Human Lipoprotein(a) Transgenic Mice Shows an Oxidative Stress and Lipid Export Response. BioMed Research International No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1127016
American Medical Association (AMA)
Rodger, Euan J.& Porteous, Carolyn M.& Jones, Gregory T.& Legge, Michael& Kleffmann, Torsten& McCormick, Sally P. A.. Proteomic Analysis of Liver from Human Lipoprotein(a) Transgenic Mice Shows an Oxidative Stress and Lipid Export Response. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1127016
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127016