Shenqi Fuzheng Injection (SFI) Enhances IFN-α Inhibitory Effect on Hepatocellular Carcinoma Cells by Reducing VEGF Expression: Validation by Gene Silencing Technique
Joint Authors
Wang, Jun-Hui
Chen, Xiaoheng
Qi, Shuo
Li, Zhe
He, Bei
Li, Hui long
Fu, Jinxiang
Huang, Sheng
Zhang, Lei
Li, Xinai
Hu, Rui
Li, Lu
Wang, Tangshun
Xue, Feng
Gao, Xiang
Shi, Xiaoguang
Zhang, Tao
Wang, Xin
Ding, Zhiguo
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-23
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Shenqi Fuzheng Injection (SFI) is a traditional Chinese medicine injection with anticancer properties and is mainly composed of ginseng and astragalus.
Its efficacy has been confirmed in clinical trials, but the mechanism remains unclear.
We investigated the effect of SFI on vascular endothelial growth factor (VEGF) gene expression in hepatocellular carcinoma (HCC) cells and identified its possible mechanism of synergistic effects when combined with the chemotherapeutic drug interferon (IFN-) α.
An MTT assay was used to measure the inhibition effects of low-dose IFN-α (6000 IU) with or without SFI (0.5 g/L) on the HCC cell line MHCC97.
VEGF-silenced MHCC97L-mir200 cell lines were prepared using lentiviral vectors and evaluated by real-time PCR to determine the inhibition effect.
We examined MHCC97L-mir200 and MHCC97L cells by MTT assay, using IFN-α alone or in combination with SFI.
The inhibition ratio of IFN-α (6000 IU) was -29.5%, while that for IFN-α (6000 IU) + SFI (0.5 g/L) was 17.0%, which was significantly higher than that for the IFN-α group (P < 0.01).
The VEGF gene was silenced successfully in MHCC97-L cells.
After interference of VEGF, the inhibition by SFI and IFN-α in MHCC97L-mir200 did not differ from that in MHCC97-L cells (P > 0.05).
SFI can reduce the expression of VEGF in HCC, which can increase the efficacy of IFN-α, providing a theoretical basis for clinical application.
American Psychological Association (APA)
Chen, Xiaoheng& Qi, Shuo& Li, Zhe& He, Bei& Li, Hui long& Fu, Jinxiang…[et al.]. 2019. Shenqi Fuzheng Injection (SFI) Enhances IFN-α Inhibitory Effect on Hepatocellular Carcinoma Cells by Reducing VEGF Expression: Validation by Gene Silencing Technique. BioMed Research International،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1127711
Modern Language Association (MLA)
Chen, Xiaoheng…[et al.]. Shenqi Fuzheng Injection (SFI) Enhances IFN-α Inhibitory Effect on Hepatocellular Carcinoma Cells by Reducing VEGF Expression: Validation by Gene Silencing Technique. BioMed Research International No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1127711
American Medical Association (AMA)
Chen, Xiaoheng& Qi, Shuo& Li, Zhe& He, Bei& Li, Hui long& Fu, Jinxiang…[et al.]. Shenqi Fuzheng Injection (SFI) Enhances IFN-α Inhibitory Effect on Hepatocellular Carcinoma Cells by Reducing VEGF Expression: Validation by Gene Silencing Technique. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1127711
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127711