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The Potential Protective Effect of Curcumin on Amyloid-β-42 Induced Cytotoxicity in HT-22 Cells
Joint Authors
Cheng, Xuan
Zhang, Lu
Lian, Ya-Jun
Fang, Yu
Zeng, Zhaoshu
Wu, Chuanjie
Zhu, Hongcan
Xu, Hongliang
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
We aimed to investigate the effect and mechanism of curcumin (CUR) in Alzheimer’s disease (AD).
Methods.
Mouse hippocampal neuronal cell line HT-22 was treated with Aβ1–42 and/or CUR, and then cell viability was evaluated by cell counting kit 8, Beclin-l level was detected using western blotting, and the formation of autophagosomes was observed by transmission electron microscopy (TEM).
Furthermore, transcriptome sequencing and analysis were performed in cells with Aβ1–42 alone or Aβ1–42 + CUR.
Results.
Aβ1–42 treatment significantly inhibited cell viability compared with untreated cells (P<0.01).
After treatment for 48 h, CUR remarkably promoted cell viability compared with cell treated with Aβ1–42 alone (P<0.01).
Compared with cells treated with Aβ1–42 alone, the expression of Beclin-1 was slightly reduced in cells with combined treatment of Aβ1–42 with CUR (P<0.05).
Consistently, TEM results showed that CUR inhibited the formation of autophagosomes in cells treated with Aβ1–42.
Furthermore, the protein-protein interaction network showed five key genes, including MYC, Cdh1, Acaca, Egr1, and CCnd1, likely involved in CUR effects.
Conclusions.
CUR might have a potential neuroprotective effect by promoting cell viability in AD, which might be associated with cell autophagy.
Furthermore, MYC, Cdh1, and Acaca might be involved in the progression of AD.
American Psychological Association (APA)
Zhang, Lu& Fang, Yu& Cheng, Xuan& Lian, Ya-Jun& Zeng, Zhaoshu& Wu, Chuanjie…[et al.]. 2018. The Potential Protective Effect of Curcumin on Amyloid-β-42 Induced Cytotoxicity in HT-22 Cells. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1128909
Modern Language Association (MLA)
Zhang, Lu…[et al.]. The Potential Protective Effect of Curcumin on Amyloid-β-42 Induced Cytotoxicity in HT-22 Cells. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1128909
American Medical Association (AMA)
Zhang, Lu& Fang, Yu& Cheng, Xuan& Lian, Ya-Jun& Zeng, Zhaoshu& Wu, Chuanjie…[et al.]. The Potential Protective Effect of Curcumin on Amyloid-β-42 Induced Cytotoxicity in HT-22 Cells. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1128909
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128909