MT1-MMP as a PET Imaging Biomarker for Pancreas Cancer Management
Joint Authors
Morcillo, Miguel Ángel
García de Lucas, Ángel
Oteo, Marta
Romero, Eduardo
Magro, Natalia
Ibáñez, Marta
Martínez, Alfonso
Garaulet, Guillermo
Arroyo, Alicia G.
López-Casas, Pedro Pablo
Hidalgo, Manuel
Mulero, Francisca
Martínez-Torrecuadrada, Jorge
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-26
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Pancreatic ductal adenocarcinoma (PDAC) continues to be one of the deadliest cancers for which optimal diagnostic tools are still greatly needed.
Identification of PDAC-specific molecular markers would be extremely useful to improve disease diagnosis and follow-up.
MT1-MMP has long been involved in pancreatic cancer, especially in tumour invasion and metastasis.
In this study, we aim to ascertain the suitability of MT1-MMP as a biomarker for positron emission tomography (PET) imaging.
Two probes were assessed and compared for this purpose, an MT1-MMP-specific binding peptide (MT1-AF7p) and a specific antibody (LEM2/15), labelled, respectively, with 68Ga and with 89Zr.
PET imaging with both probes was conducted in patient-derived xenograft (PDX), subcutaneous and orthotopic, PDAC mouse models, and in a cancer cell line (CAPAN-2)-derived xenograft (CDX) model.
Both radiolabelled tracers were successful in identifying, by means of PET imaging techniques, tumour tissues expressing MT1-MMP although they did so at different uptake levels.
The 89Zr-DFO-LEM2/15 probe showed greater specific activity compared to the 68Ga-labelled peptide.
The mean value of tumour uptake for the 89Zr-DFO-LEM2/15 probe (5.67 ± 1.11%ID/g, n=28) was 25–30 times higher than that of the 68Ga-DOTA-AF7p ones.
Tumour/blood ratios (1.13 ± 0.51 and 1.44 ± 0.43 at 5 and 7 days of 89Zr-DFO-LEM2/15 after injection) were higher than those estimated for 68Ga-DOTA-AF7p probes (of approximately tumour/blood ratio = 0.5 at 90 min after injection).
Our findings strongly point out that (i) the in vivo detection of MT1-MMP by PET imaging is a promising strategy for PDAC diagnosis and (ii) labelled LEM2/15 antibody is a better candidate than MT1-AF7p for PDAC detection.
American Psychological Association (APA)
Morcillo, Miguel Ángel& García de Lucas, Ángel& Oteo, Marta& Romero, Eduardo& Magro, Natalia& Ibáñez, Marta…[et al.]. 2018. MT1-MMP as a PET Imaging Biomarker for Pancreas Cancer Management. Contrast Media & Molecular Imaging،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1131581
Modern Language Association (MLA)
Morcillo, Miguel Ángel…[et al.]. MT1-MMP as a PET Imaging Biomarker for Pancreas Cancer Management. Contrast Media & Molecular Imaging No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1131581
American Medical Association (AMA)
Morcillo, Miguel Ángel& García de Lucas, Ángel& Oteo, Marta& Romero, Eduardo& Magro, Natalia& Ibáñez, Marta…[et al.]. MT1-MMP as a PET Imaging Biomarker for Pancreas Cancer Management. Contrast Media & Molecular Imaging. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1131581
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1131581