Cloning and Functional Determination of Ammonium Transporter PpeAMT3;4 in Peach

Joint Authors

Wu, Zheng
Tan, Ping
Wang, Yuqing
You, Shuanghong
Li, Yanju
Li, Yuhe
Shi, Wenjing
Song, Zhizhong

Source

BioMed Research International

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-12-04

Country of Publication

Egypt

No. of Pages

7

Main Subjects

Medicine

Abstract EN

Ammonium (NH4+) plays key roles in plant growth, development, fruit quality, and yield.

In plants, NH4+ uptake and transport are facilitated by NH4+ transporters (AMT).

However, molecular mechanisms and physiological functions of type-II AMT (AMT2) transporters in fruit trees are still unclear, especially in peach.

In this study, we cloned and characterized an AMT2 family gene from peach, PpeAMT3;4, and determined its function in yeast mutant.

Expression analysis showed that PpeAMT3;4 was majorly expressed in peach roots and significantly decreased by NH4+ excess but had no response to NH4+ deficiency.

Functional determination and 15nitrogen-labeled NH4+ uptake assay in yeast cells implied that PpeAMT3;4 was a typical high-affinity transporter, with a Km value of 86.3 μM, that can uptake external NH4+ in yeast cells.

This study provides gene resources to uncover the biological function of AMT2 transporters and reveals molecular basis for NH4+ uptake and nitrogen (N) nutrition mechanisms in fruit trees.

American Psychological Association (APA)

You, Shuanghong& Wang, Yuqing& Li, Yanju& Li, Yuhe& Tan, Ping& Wu, Zheng…[et al.]. 2020. Cloning and Functional Determination of Ammonium Transporter PpeAMT3;4 in Peach. BioMed Research International،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1132346

Modern Language Association (MLA)

You, Shuanghong…[et al.]. Cloning and Functional Determination of Ammonium Transporter PpeAMT3;4 in Peach. BioMed Research International No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1132346

American Medical Association (AMA)

You, Shuanghong& Wang, Yuqing& Li, Yanju& Li, Yuhe& Tan, Ping& Wu, Zheng…[et al.]. Cloning and Functional Determination of Ammonium Transporter PpeAMT3;4 in Peach. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1132346

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1132346