Cloning and Functional Determination of Ammonium Transporter PpeAMT3;4 in Peach
Joint Authors
Wu, Zheng
Tan, Ping
Wang, Yuqing
You, Shuanghong
Li, Yanju
Li, Yuhe
Shi, Wenjing
Song, Zhizhong
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-04
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Ammonium (NH4+) plays key roles in plant growth, development, fruit quality, and yield.
In plants, NH4+ uptake and transport are facilitated by NH4+ transporters (AMT).
However, molecular mechanisms and physiological functions of type-II AMT (AMT2) transporters in fruit trees are still unclear, especially in peach.
In this study, we cloned and characterized an AMT2 family gene from peach, PpeAMT3;4, and determined its function in yeast mutant.
Expression analysis showed that PpeAMT3;4 was majorly expressed in peach roots and significantly decreased by NH4+ excess but had no response to NH4+ deficiency.
Functional determination and 15nitrogen-labeled NH4+ uptake assay in yeast cells implied that PpeAMT3;4 was a typical high-affinity transporter, with a Km value of 86.3 μM, that can uptake external NH4+ in yeast cells.
This study provides gene resources to uncover the biological function of AMT2 transporters and reveals molecular basis for NH4+ uptake and nitrogen (N) nutrition mechanisms in fruit trees.
American Psychological Association (APA)
You, Shuanghong& Wang, Yuqing& Li, Yanju& Li, Yuhe& Tan, Ping& Wu, Zheng…[et al.]. 2020. Cloning and Functional Determination of Ammonium Transporter PpeAMT3;4 in Peach. BioMed Research International،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1132346
Modern Language Association (MLA)
You, Shuanghong…[et al.]. Cloning and Functional Determination of Ammonium Transporter PpeAMT3;4 in Peach. BioMed Research International No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1132346
American Medical Association (AMA)
You, Shuanghong& Wang, Yuqing& Li, Yanju& Li, Yuhe& Tan, Ping& Wu, Zheng…[et al.]. Cloning and Functional Determination of Ammonium Transporter PpeAMT3;4 in Peach. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1132346
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1132346