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Culture of iPSCs Derived Pancreatic β-Like Cells In Vitro Using Decellularized Pancreatic Scaffolds: A Preliminary Trial
Joint Authors
Yu-Hua, Lu
Zhi-Wei, Wang
Yao, Wang
Wan, Jian
Guo, Yibing
Huang, Yan
Zhou, Pengcheng
Wu, Cen
Zhu, Shajun
Wang, Lei
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-05
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Diabetes mellitus is a disease which has affected 415 million patients in 2015.
In an effort to replace the significant demands on transplantation and morbidity associated with transplantation, the production of β-like cells differentiated from induced pluripotent stem cells (iPSCs) was evaluated.
This approach is associated with promising decellularized scaffolds with natural extracellular matrix (ECM) and ideal cubic environment that will promote cell growth in vivo.
Our efforts focused on combining decellularized rat pancreatic scaffolds with mouse GFP+-iPSCs-derived pancreatic β-like cells, to evaluate whether decellularized scaffolds could facilitate the growth and function of β-like cells.
β-like cells were differentiated from GFP+-iPSCs and evaluated via cultivating in the dynamic circulation perfusion device.
Our results demonstrated that decellularized pancreatic scaffolds display favorable biochemical properties.
Furthermore, not only could the scaffolds support the survival of β-like cells, but they also accelerated the expression of the insulin as compared to plate-based cell culture.
In conclusion, these results suggest that decellularized pancreatic scaffolds could provide a suitable platform for cellular activities of β-like cells including survival and insulin secretion.
This study provides preliminary support for regenerating insulin-secreting organs from the decellularized scaffolds combined with iPSCs derived β-like cells as a potential clinical application.
American Psychological Association (APA)
Wan, Jian& Huang, Yan& Zhou, Pengcheng& Guo, Yibing& Wu, Cen& Zhu, Shajun…[et al.]. 2017. Culture of iPSCs Derived Pancreatic β-Like Cells In Vitro Using Decellularized Pancreatic Scaffolds: A Preliminary Trial. BioMed Research International،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1136938
Modern Language Association (MLA)
Wan, Jian…[et al.]. Culture of iPSCs Derived Pancreatic β-Like Cells In Vitro Using Decellularized Pancreatic Scaffolds: A Preliminary Trial. BioMed Research International No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1136938
American Medical Association (AMA)
Wan, Jian& Huang, Yan& Zhou, Pengcheng& Guo, Yibing& Wu, Cen& Zhu, Shajun…[et al.]. Culture of iPSCs Derived Pancreatic β-Like Cells In Vitro Using Decellularized Pancreatic Scaffolds: A Preliminary Trial. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1136938
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1136938