Identification of Vinculin as a Potential Diagnostic Biomarker for Acute Aortic Dissection Using Label-Free Proteomics
Joint Authors
Wang, He-Qing
Yang, Hang
Tang, Qian
Gong, Yi-Chen
Fu, Yuan-Hao
Wan, Feng
Yang, Bo
Guo, Rui
Zhong, Yong-Liang
Zhu, Jun-Ming
Zhang, Zhe
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-20
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Acute aortic dissection (AAD) is an emergent vascular disease.
Currently, its diagnosis depends on clinical and radiological investigations but lacking of serum biomarkers.
In this study, we aimed to identify potential serum biomarkers for AAD using label-free proteomics approach.
A total of 90 serum samples were collected from three groups: patients with acute aortic dissection (AAD, n=30), patients with acute myocardial infarction (AMI, n=30), and healthy controls (n=30), and the first four samples from each group were selected for label-free proteomics analysis.
Using label-free approach, a total of 22 differentially expressed proteins were identified in the serum samples of the AAD group, of which 15 were upregulated and 7 were downregulated as compared to the AMI and healthy control groups.
The most prominent increased protein was vinculin, which was selected to validate in total samples.
The level of vinculin was significantly elevated in AAD patients (15.8 ng/ml, IQR: 9.3-19.9 ng/ml) than that in AMI patients (8.6 ng/ml, IQR:5.3-11.4 ng/ml) and healthy volunteers (5.3 ng/ml, IQR:2.8-7.6 ng/ml), P<0.0001.
Furthermore, the concentration of vinculin both increased in type A and B dissection.
At the early stage of AAD, vinculin maintained a high level to 48 hours compared with that of AMI.
Our study demonstrated that vinculin may play a role in the early diagnosis of AAD.
American Psychological Association (APA)
Wang, He-Qing& Yang, Hang& Tang, Qian& Gong, Yi-Chen& Fu, Yuan-Hao& Wan, Feng…[et al.]. 2020. Identification of Vinculin as a Potential Diagnostic Biomarker for Acute Aortic Dissection Using Label-Free Proteomics. BioMed Research International،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1137162
Modern Language Association (MLA)
Wang, He-Qing…[et al.]. Identification of Vinculin as a Potential Diagnostic Biomarker for Acute Aortic Dissection Using Label-Free Proteomics. BioMed Research International No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1137162
American Medical Association (AMA)
Wang, He-Qing& Yang, Hang& Tang, Qian& Gong, Yi-Chen& Fu, Yuan-Hao& Wan, Feng…[et al.]. Identification of Vinculin as a Potential Diagnostic Biomarker for Acute Aortic Dissection Using Label-Free Proteomics. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1137162
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137162