Characterisation of Casein Kinase 1.1 in Leishmania donovani Using the CRISPR Cas9 Toolkit
Joint Authors
Martel, Daniel
Beneke, Tom
Gluenz, Eva
Späth, Gerald F.
Rachidi, Najma
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-29
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The recent adaptation of CRISPR Cas9 genome editing to Leishmania spp.
has opened a new era in deciphering Leishmania biology.
The method was recently improved using a PCR-based CRISPR Cas9 approach, which eliminated the need for cloning.
This new approach, which allows high-throughput gene deletion, was successfully validated in L.
mexicana and L.
major.
In this study, we validated the toolkit in Leishmania donovani targeting the flagellar protein PF16, confirming that the tagged protein localizes to the flagellum and that null mutants lose their motility.
We then used the technique to characterise CK1.1, a member of the casein kinase 1 family, which is involved in the regulation of many cellular processes.
We showed that CK1.1 is a low-abundance protein present in promastigotes and in amastigotes.
We demonstrated that CK1.1 is not essential for promastigote and axenic amastigote survival or for axenic amastigote differentiation, although it may have a role during stationary phase.
Altogether, our data validate the use of PCR-based CRISPR Cas9 toolkit in L.
donovani, which will be crucial for genetic modification of hamster-derived, disease-relevant parasites.
American Psychological Association (APA)
Martel, Daniel& Beneke, Tom& Gluenz, Eva& Späth, Gerald F.& Rachidi, Najma. 2017. Characterisation of Casein Kinase 1.1 in Leishmania donovani Using the CRISPR Cas9 Toolkit. BioMed Research International،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1137223
Modern Language Association (MLA)
Martel, Daniel…[et al.]. Characterisation of Casein Kinase 1.1 in Leishmania donovani Using the CRISPR Cas9 Toolkit. BioMed Research International No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1137223
American Medical Association (AMA)
Martel, Daniel& Beneke, Tom& Gluenz, Eva& Späth, Gerald F.& Rachidi, Najma. Characterisation of Casein Kinase 1.1 in Leishmania donovani Using the CRISPR Cas9 Toolkit. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1137223
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137223