Online Detection of Peroxidase Using 3D Printing, Active Magnetic Mixing, and Spectra Analysis
Joint Authors
Bai, Shanshan
Gan, Chengqi
Cai, Gaozhe
Wang, Lei
Chen, Mingyong
Han, Qingan
Lin, Jianhan
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A new method for online detection of peroxidase (POD) using 3D printing, active magnetic mixing, fluidic control, and optical detection was developed and demonstrated in this study.
The proposed POD detection system consisted of a 3D printing and active magnetic mixing based fluidic chip for online catalytic reaction, an optical detector with a fluidic flow cell for quantitative determination of the final catalysate, and a single-chip microcontroller based controller for automatic control of two rotating magnetic fields and four precise peristaltic pumps.
Horseradish peroxidase (HRP) was used as research model and a linear relationship between the absorbance at the characteristic wavelength of 450 nm and the concentration of HRP of 1/4–1/128 μg mL−1 was obtained as A = 0.257ln(C) + 1.425 (R2 = 0.976).
For the HRP spiked pork tests, the recoveries of HRP ranged from 93.5% to 110.4%, indicating that this proposed system was capable of detecting HRP in real samples.
It has the potential to be extended for online detection of the activity of other enzymes and integration with ELISA method for biological and chemical analysis.
American Psychological Association (APA)
Bai, Shanshan& Gan, Chengqi& Cai, Gaozhe& Wang, Lei& Chen, Mingyong& Han, Qingan…[et al.]. 2017. Online Detection of Peroxidase Using 3D Printing, Active Magnetic Mixing, and Spectra Analysis. BioMed Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1137386
Modern Language Association (MLA)
Bai, Shanshan…[et al.]. Online Detection of Peroxidase Using 3D Printing, Active Magnetic Mixing, and Spectra Analysis. BioMed Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1137386
American Medical Association (AMA)
Bai, Shanshan& Gan, Chengqi& Cai, Gaozhe& Wang, Lei& Chen, Mingyong& Han, Qingan…[et al.]. Online Detection of Peroxidase Using 3D Printing, Active Magnetic Mixing, and Spectra Analysis. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1137386
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137386