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Revealing PAK2’s Function in the Cell Division through MKLP1’s Interactome
Joint Authors
Zhang, Zhao-huan
Liu, Xiu-ling
Zhu, Yun-yi
Huang, Hai
Xu, Xiao-hui
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-06
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Cell division-related proteins are essential for the normal development and differentiation of cells and may be related to the occurrence of cancer and the drug resistance mechanism of cancer cells.
The mitotic kinesin-like protein 1 (MKLP1) is a kinesin protein that has been involved in the assembly of the midzone/midbody during mitosis and cytokinesis.
In this study, we found that the tail domain of MKLP1 exhibited an autoinhibitory effect on its motor activity.
Overexpression of the tail domain in HEK293 cells blocked cytokinesis and caused bi-/multinucleation.
It is possible that protein binding to the MKLP1 tail relieves this autoinhibition and induces the motility of MKLP1.
We used the GST pull-down assay followed by the LC-MS/MS analysis and identified 54 MKLP1 tail domain-specific binding proteins.
Further, we confirmed the MS result by coimmunoprecipitation and FRET that a serine/threonine kinase, p21-activated kinase 2 (PAK2), binding to MKLP1.
Endogenous PAK2 expression was found to be identical to that of MKLP1 in HEK293 cells during cytokinesis.
Finally, functional studies indicated that when PAK2 expression was downregulated by siRNA, MKLP1 underwent a change in its localization away from the midbody, and cell cytokinesis was subsequently impeded.
This study presents a novel regulatory mechanism that PAK2 promotes the activation of MKLP1 and contributes to complete cell cytokinesis.
American Psychological Association (APA)
Zhang, Zhao-huan& Liu, Xiu-ling& Zhu, Yun-yi& Huang, Hai& Xu, Xiao-hui. 2020. Revealing PAK2’s Function in the Cell Division through MKLP1’s Interactome. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1137773
Modern Language Association (MLA)
Zhang, Zhao-huan…[et al.]. Revealing PAK2’s Function in the Cell Division through MKLP1’s Interactome. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1137773
American Medical Association (AMA)
Zhang, Zhao-huan& Liu, Xiu-ling& Zhu, Yun-yi& Huang, Hai& Xu, Xiao-hui. Revealing PAK2’s Function in the Cell Division through MKLP1’s Interactome. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1137773
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137773