Effects of Fungicides on Rat’s Neurosteroid Synthetic Enzymes
Joint Authors
Ge, Ren-Shan
Chen, Fan
Shen, Xiuwei
Huang, Peng
Chen, Lanlan
Su, Ying
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Exposure to environmental endocrine disruptors may interfere with nervous system’s activity.
Fungicides such as tebuconazole, triadimefon, and vinclozolin have antifungal activities and are used to prevent fungal infections in agricultural plants.
In the present study, we studied effects of tebuconazole, triadimefon, and vinclozolin on rat’s neurosteroidogenic 5α-reductase 1 (5α-Red1), 3α-hydroxysteroid dehydrogenase (3α-HSD), and retinol dehydrogenase 2 (RDH2).
Rat’s 5α-Red1, 3α-HSD, and RDH2 were cloned and expressed in COS-1 cells, and effects of these fungicides on them were measured.
Tebuconazole and triadimefon competitively inhibited 5α-Red1, with IC50 values of 8.670 ± 0.771 × 10−6 M and 17.390 ± 0.079 × 10−6 M, respectively, while vinclozolin did not inhibit the enzyme at 100 × 10−6 M.
Triadimefon competitively inhibited 3α-HSD, with IC50 value of 26.493 ± 0.076 × 10−6 M.
Tebuconazole and vinclozolin weakly inhibited 3α-HSD, with IC50 values about 100 × 10−6 M, while vinclozolin did not inhibit the enzyme even at 100 × 10−6 M.
Tebuconazole and triadimefon weakly inhibited RDH2 with IC50 values over 100 × 10−6 M and vinclozolin did not inhibit this enzyme at 100 × 10−6 M.
Docking study showed that tebuconazole, triadimefon, and vinclozolin bound to the steroid-binding pocket of 3α-HSD.
In conclusion, triadimefon potently inhibited rat’s neurosteroidogenic enzymes, 5α-Red1 and 3α-HSD.
American Psychological Association (APA)
Shen, Xiuwei& Chen, Fan& Chen, Lanlan& Su, Ying& Huang, Peng& Ge, Ren-Shan. 2017. Effects of Fungicides on Rat’s Neurosteroid Synthetic Enzymes. BioMed Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1137795
Modern Language Association (MLA)
Shen, Xiuwei…[et al.]. Effects of Fungicides on Rat’s Neurosteroid Synthetic Enzymes. BioMed Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1137795
American Medical Association (AMA)
Shen, Xiuwei& Chen, Fan& Chen, Lanlan& Su, Ying& Huang, Peng& Ge, Ren-Shan. Effects of Fungicides on Rat’s Neurosteroid Synthetic Enzymes. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1137795
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137795