Exploring the Antitumor Mechanisms of Zingiberis Rhizoma Combined with Coptidis Rhizoma Using a Network Pharmacology Approach
Joint Authors
Wang, Meng
Qi, Youke
Sun, Yongning
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-28
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
Background.
Although the combination of Zingiberis rhizoma (ZR) and Coptidis rhizoma (CR) is a classic traditional Chinese medicine-based herbal pair used for its antitumor effect, the material basis and underlying mechanisms are unclear.
Here, a network pharmacology approach was used to elucidate the antitumor mechanisms of ZR-CR.
Materials and Methods.
To predict the targets of ZR-CR in treating tumors, we constructed protein–protein interactions and hub component-target networks and performed pathway and process enrichment and molecular docking analysis.
We used a surface plasmon resonance (SPR) assay to validate the predicted component-target affinities.
Hub gene expression and survival analysis in patients with tumors were used to predict the clinical significance.
Results.
The active components of ZR-CR—shogaol, daucosterol, ginkgetin, berberine, quercetin, chlorogenic acid, and vanillic acid—exhibited antitumor activities via the MAPK, PI3K-AKT, TNF, FOXO, HIF-1, and VEGF signaling pathways.
Molecular docking and SPR analyses suggested direct binding of berberine with AKT1 and TP53; quercetin with EGFR and VEGF165; and ginkgetin, isoginkgetin, and daucosterol with VEGF165 with weak affinities.
Gene expression levels of the hub targets of ZR-CR were associated with overall survival and disease-free survival in patients with various tumor types.
Conclusions.
The antitumor components of the ZR-CR herbal pair and the mechanisms underlying their antitumor effects were identified.
These antitumor components deserve to be explored further in experimental and clinical studies.
American Psychological Association (APA)
Wang, Meng& Qi, Youke& Sun, Yongning. 2020. Exploring the Antitumor Mechanisms of Zingiberis Rhizoma Combined with Coptidis Rhizoma Using a Network Pharmacology Approach. BioMed Research International،Vol. 2020, no. 2020, pp.1-18.
https://search.emarefa.net/detail/BIM-1137822
Modern Language Association (MLA)
Wang, Meng…[et al.]. Exploring the Antitumor Mechanisms of Zingiberis Rhizoma Combined with Coptidis Rhizoma Using a Network Pharmacology Approach. BioMed Research International No. 2020 (2020), pp.1-18.
https://search.emarefa.net/detail/BIM-1137822
American Medical Association (AMA)
Wang, Meng& Qi, Youke& Sun, Yongning. Exploring the Antitumor Mechanisms of Zingiberis Rhizoma Combined with Coptidis Rhizoma Using a Network Pharmacology Approach. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-18.
https://search.emarefa.net/detail/BIM-1137822
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137822