Deletion of Bmal1 Impairs Pancreatic β-Cell Function via Mitochondrial Signaling Pathway
Joint Authors
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-07
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Several studies have demonstrated that brain and muscle Arnt-like protein-1 (Bmal1) acts as a core clock gene for maintaining normal cell function, including hepatocytes and cardiomyocytes.
Loss of Bmal1 is associated with type 2 diabetes due to pancreatic β-cell failure.
However, little information is available about its role and mechanism in pancreatic β-cell.
To address this, we investigated the consequences of Bmal1 inhibition in an insulinoma cell line (INS-1) by using small interfering RNA (siRNA).
We observed that knockout of Bmal1 impaired glucose-stimulated insulin secretion in β-cell.
Meanwhile, the depletion of Bmal1 in β-cell caused an adverse change in mitochondrial membrane potential and mitochondrial architecture.
Deletion of Bmal1 attenuated mRNA and protein expression of mitofusin 1 (Mfn1) and mitofusin 2 (Mfn2) and enhanced the expression of fission 1 (Fis1).
In summary, the deletion of Bmal1 impaired β-cell function may be via the mitochondrial signaling pathway in INS-1 cells.
American Psychological Association (APA)
Ye, Lu& Wu, Huaxiang& Xu, Weihong. 2020. Deletion of Bmal1 Impairs Pancreatic β-Cell Function via Mitochondrial Signaling Pathway. BioMed Research International،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1138302
Modern Language Association (MLA)
Ye, Lu…[et al.]. Deletion of Bmal1 Impairs Pancreatic β-Cell Function via Mitochondrial Signaling Pathway. BioMed Research International No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1138302
American Medical Association (AMA)
Ye, Lu& Wu, Huaxiang& Xu, Weihong. Deletion of Bmal1 Impairs Pancreatic β-Cell Function via Mitochondrial Signaling Pathway. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1138302
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138302